In Vitro Fermentation of Monosodium Glutamate with Human Gut Microbes

doi:10.13560/j.cnki.biotech.bull.1985.2020-0600

Abstract

Abstract:

This study aims to investigate the effects of monosodium glutamate on the composition and functions of human gut microbes. In vitro fermentation of gut microbes using sodium glutamate as substrate was conducted,and the content of short-chain fatty acids（SCFA）and γ-aminobutyric acid（GABA）in the fermentation broth was determined. The results showed that the total SCFA yield in the fermentation with monosodium glutamate was significantly higher than the control without substrate（P<0.01）,and the content of butyric acid increased by 26 times. The conversion rate of monosodium glutamate reached 72.36% after in vitro fermentation. The results from in vitro fermentation with monosodium glutamate showed that the relative abundance of Bacteroidete and Proteobateria phyla in vitro fermentation with monosodium glutamate increased（P<0.05）if compared with the original feces,while that of Firmicutes and Actinobacteria phyla decreased（P<0.01）. Escherichia-Shigella and Bacteroides genera increased（P<0.01）while monosodium glutamate was added. Our results indicate that in vitro fermentation with monosodium glutamate increase the content of SCFAs in fermentation broths,especially the butyric acid,and affect the composition and function of human gut microbes;moreover,monosodium glutamate may convert into GABA via in vitro fermentation with gut microbes.

Key words: glutamate, in vitro fermentation, gut microbes, γ-aminobutyric acid;, short-chain fatty acid

LIU Shu-jun, CHEN Miao, WANG Feng-zhong, BAO Yu-ming, XIN Feng-jiao, WEN Bo-ting. In Vitro Fermentation of Monosodium Glutamate with Human Gut Microbes[J]. Biotechnology Bulletin, 2020, 36(12): 104-112.

Figures/Tables 8

References 26

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项目/（mmol·L^-1）	CK	FS	P
总酸	13.37±0.67	62.26±10.91	<0.01
乙酸	10.47±0.31	47.73±9.54	<0.01
丙酸	1.69±0.27	5.79±0.39	<0.01
丁酸	0.24±0.02	6.28±1.92	<0.01
异丁酸	0.25±0.09	0.30±0.13	0.74
戊酸	0.04±0.01	1.02±0.72	0.19
异戊酸	0.67±0.25	1.12±0.48	0.42

项目/（mmol·L^-1）	CK	FS	P
总酸	13.37±0.67	62.26±10.91	<0.01
乙酸	10.47±0.31	47.73±9.54	<0.01
丙酸	1.69±0.27	5.79±0.39	<0.01
丁酸	0.24±0.02	6.28±1.92	<0.01
异丁酸	0.25±0.09	0.30±0.13	0.74
戊酸	0.04±0.01	1.02±0.72	0.19
异戊酸	0.67±0.25	1.12±0.48	0.42