Isolation and Identification of Bacillus amyloliquefaciens T-6 and Its Potential of Resisting Disease and Promoting Growth

doi:10.13560/j.cnki.biotech.bull.1985.2020-0847

Abstract

Abstract: One Bacillus strain with antagonistic effect on peach root rot and owned a wide antibacterial range was isolated in situ from the peach orchard in Sihong,Jiangsu province. After analysis in morphology,Biolog physiology and biochemistry,16S rDNA sequence and specific PCR,the strain was identified as Bacillus amyloliquefaciens. The enzyme activity of bacteriostatic substance and growth promotion performance test revealed that the T-6 strain had the ability of producing protease,chitinase,as well as the ability of phosphorus-dissolving,phosphate-resolving and potassium-dissolving. In the pot experiment,the dry weight,total root length,and chlorophyll ratio of tomato seedlings in the T-6 treatment group increased by 31.81%,50.79%,and 35.11%,respectively,and the control effect reached 54.69%. Therefore,it is concluded that T-6 strain has great potential in biological control of soil-borne diseases such as peach root rot.

Key words: Fusarium solani, Bacillus spp., peach root rot, biological control.

YAO Ya-qian, CHENG Na-na, LI Pei-gen, LU Yi, WANG Yan-gang, LIN Rong-shan, ZHOU Bo, WANG Bing. Isolation and Identification of Bacillus amyloliquefaciens T-6 and Its Potential of Resisting Disease and Promoting Growth[J]. Biotechnology Bulletin, 2020, 36(9): 202-210.

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