Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 40-50.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0872
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ZHANG Ya-jing1,2(), SONG Mei-yan1,2, ZHANG Yi-jing2,3, FANG Qing2, YANG Jun3, PENG De-liang2, HUANG Wen-kun2, PENG Huan2, ZHU Ying-bo1(), KONG Ling-an2()
Received:
2020-07-15
Online:
2021-02-26
Published:
2021-02-26
Contact:
ZHU Ying-bo,KONG Ling-an
E-mail:1748882140@qq.com;zhu-yb@126.com;konglingan@caas.cn
ZHANG Ya-jing, SONG Mei-yan, ZHANG Yi-jing, FANG Qing, YANG Jun, PENG De-liang, HUANG Wen-kun, PENG Huan, ZHU Ying-bo, KONG Ling-an. Identification of Purpureocillium lilacinum and Trichoderma harzianum Strains for Simultaneously Controlling Cucumber Root Rot and Root-knot Nematode Diseases[J]. Biotechnology Bulletin, 2021, 37(2): 40-50.
采样地点 | 作物 | 样品数量 | 分离菌株数量 |
---|---|---|---|
河北秦皇岛 | 番茄 | 1 | 52 |
河北张家口 | 黄瓜 | 1 | 63 |
内蒙古呼和浩特 | 黄瓜 | 3 | 75 |
内蒙古赤峰 | 番茄 | 2 | 59 |
内蒙古呼和浩特 | 番茄 | 1 | 43 |
辽宁朝阳 | 黄瓜 | 4 | 92 |
辽宁沈阳 | 番茄 | 5 | 128 |
黑龙江黑河 | 番茄 | 1 | 15 |
黑龙江大庆 | 黄瓜 | 2 | 32 |
黑龙江黑河 | 黄瓜 | 1 | 22 |
黑龙江黑河 | 辣椒 | 1 | 20 |
黑龙江黑河 | 马铃薯 | 1 | 23 |
黑龙江黑河 | 茄子 | 1 | 23 |
吉林白城 | 马铃薯 | 1 | 52 |
合计 | 25 | 699 |
采样地点 | 作物 | 样品数量 | 分离菌株数量 |
---|---|---|---|
河北秦皇岛 | 番茄 | 1 | 52 |
河北张家口 | 黄瓜 | 1 | 63 |
内蒙古呼和浩特 | 黄瓜 | 3 | 75 |
内蒙古赤峰 | 番茄 | 2 | 59 |
内蒙古呼和浩特 | 番茄 | 1 | 43 |
辽宁朝阳 | 黄瓜 | 4 | 92 |
辽宁沈阳 | 番茄 | 5 | 128 |
黑龙江黑河 | 番茄 | 1 | 15 |
黑龙江大庆 | 黄瓜 | 2 | 32 |
黑龙江黑河 | 黄瓜 | 1 | 22 |
黑龙江黑河 | 辣椒 | 1 | 20 |
黑龙江黑河 | 马铃薯 | 1 | 23 |
黑龙江黑河 | 茄子 | 1 | 23 |
吉林白城 | 马铃薯 | 1 | 52 |
合计 | 25 | 699 |
省份 | 菌株编号 | 分子鉴定结果 | 土样来源作物 | 安全性 |
---|---|---|---|---|
河北 | 2018-40 | Aspergillus spp. | 番茄 | 黄化萎蔫 |
2018-41 | Aspergillus ochraceopetaliformis | 番茄 | 干枯 | |
2018-42 | Aspergillus ochraceopetaliformis | 番茄 | 安全 | |
2018-43 | Aspergillus insulicola | 番茄 | 黄化萎蔫 | |
2018-44 | Trichoderma harzianum | 辣椒 | 安全 | |
2018-45 | Aspergillus ochraceus | 番茄 | 黄化萎蔫 | |
2018-46 | Aspergillus ochraceus | 番茄 | 黄化萎蔫 | |
2018-47 | Myrothecium verrucaria | 番茄 | 干枯 | |
2018-48 | Aspergillus ochraceopetaliformis | 番茄 | 干枯 | |
2018-49 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-50 | Fusarium spp. | 番茄 | 黄化萎蔫 | |
2018-51 | Penicillium simplicissimum | 番茄 | 安全 | |
2018-52 | Metacordyceps chlamydosporia | 番茄 | 安全 | |
2018-53 | Aspergillus ochraceus | 番茄 | 干枯 | |
2018-54 | Trichoderma harzianum | 辣椒 | 安全 | |
2018-83 | Aspergillus ochraceus | 黄瓜 | 黄化萎蔫 | |
2018-84 | Aspergillus ochraceus | 黄瓜 | 干枯 | |
2018-85 | Chaetomium spp. | 黄瓜 | 黄化萎蔫 | |
2018-86 | Aspergillus ochraceus | 黄瓜 | 黄化萎蔫 | |
2018-87 | Aspergillus ochraceus | 黄瓜 | 黄化萎蔫 | |
2018-88 | Aspergillus micronesiensis | 黄瓜 | 安全 | |
2018-89 | Fusarium solani | 黄瓜 | 黄化萎蔫 | |
黑龙江 | 2018-56 | Fusarium acuminatum | 番茄 | 干枯 |
2018-58 | Fusarium solani | 茄子 | 黄化萎蔫 | |
2018-75 | Fusarium solani | 茄子 | 干枯 | |
2018-76 | Fusarium oxysporum | 辣椒 | 干枯 | |
2018-77 | Fusarium solani | 辣椒 | 黄化萎蔫 | |
2018-78 | Fusarium solani | 辣椒 | 干枯 | |
2018-82 | Fusarium oxysporum | 香瓜 | 黄化萎蔫 | |
吉林 | 2018-57 | Fusarium solani | 马铃薯 | 黄化萎蔫 |
2018-59 | Chaetomium spp. | 马铃薯 | 黄化萎蔫 | |
2018-73 | Fusarium fujikuroi | 马铃薯 | 干枯 | |
2018-74 | Fusarium solani | 马铃薯 | 安全 | |
2018-79 | Purpureocillium lilacinum | 马铃薯 | 黄化萎蔫 | |
2018-80 | Chaetomium piluliferum | 马铃薯 | 安全 | |
2018-81 | Fusarium solani | 马铃薯 | 黄化萎蔫 | |
辽宁 | 2018-55 | Simplicillium obclavatum | 黄瓜 | 黄化萎蔫 |
2018-60 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-61 | Mortierella spp. | 黄瓜 | 黄化萎蔫 | |
2018-62 | Mortierella spp. | 黄瓜 | 安全 | |
2018-63 | Fusarium oxysporum | 黄瓜 | 干枯 | |
2018-64 | Mortierella spp. | 黄瓜 | 安全 | |
2018-65 | Chaetomium piluliferum | 番茄 | 安全 | |
2018-66 | Fusarium oxysporum | 黄瓜 | 黄化萎蔫 | |
2018-67 | Fusarium oxysporum | 黄瓜 | 干枯 | |
2018-68 | Mortierella alpina | 黄瓜 | 安全 | |
2018-69 | Fusarium redolens | 黄瓜 | 黄化萎蔫 | |
2018-70 | Fusarium solani | 黄瓜 | 干枯 | |
2018-71 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-72 | Chaetomium fuscum | 番茄 | 黄化萎蔫 | |
内蒙古 | 2018-1 | Fusarium solani | 马铃薯 | 干枯 |
2018-2 | Fusarium oxysporum | 马铃薯 | 黄化萎蔫 | |
2018-3 | Fusarium solani | 马铃薯 | 干枯 | |
2018-4 | Fusarium equiseti | 马铃薯 | 安全 | |
2018-5 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-6 | Beauveria bassiana | 番茄 | 安全 | |
2018-7 | Aspergillus flavipes | 黄瓜 | 安全 | |
2018-8 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-9 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-10 | Fusarium equiseti | 番茄 | 安全 | |
2018-11 | Fusarium spp. | 黄瓜 | 黄化萎蔫 | |
2018-12 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-13 | Chaetomium piluliferum | 番茄 | 黄化萎蔫 | |
2018-14 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-15 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-16 | Fusarium solani | 黄瓜 | 黄化萎蔫 | |
2018-17 | Fusarium solani | 黄瓜 | 黄化萎蔫 | |
2018-18 | Fusarium solani | 黄瓜 | 安全 | |
2018-19 | Fusarium solani | 黄瓜 | 黄化萎蔫 | |
2018-20 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-21 | Chaetomium piluliferum | 黄瓜 | 安全 | |
2018-22 | Fusarium cf. fujikuroi | 黄瓜 | 黄化萎蔫 | |
2018-23 | Albifimbria verrucaria | 番茄 | 安全 | |
2018-24 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-25 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-26 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-27 | Fusarium cf. equiseti | 番茄 | 黄化萎蔫 | |
2018-28 | Fusarium redolens | 番茄 | 干枯 | |
2018-29 | Fusarium oxysporum | 黄瓜 | 干枯 | |
2018-30 | Fusarium oxysporum | 番茄 | 黄化萎蔫 | |
2018-31 | Purpureocillium lilacinum | 番茄 | 黄化萎蔫 | |
2018-32 | Purpureocillium lilacinum | 番茄 | 安全 | |
2018-33 | Mortierella indohii | 番茄 | 黄化萎蔫 | |
2018-34 | Chaetomium piluliferum | 番茄 | 安全 | |
2018-35 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-36 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-37 | Chaetomium piluliferum | 番茄 | 安全 | |
2018-38 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-39 | Mortierella alpina | 黄瓜 | 干枯 |
省份 | 菌株编号 | 分子鉴定结果 | 土样来源作物 | 安全性 |
---|---|---|---|---|
河北 | 2018-40 | Aspergillus spp. | 番茄 | 黄化萎蔫 |
2018-41 | Aspergillus ochraceopetaliformis | 番茄 | 干枯 | |
2018-42 | Aspergillus ochraceopetaliformis | 番茄 | 安全 | |
2018-43 | Aspergillus insulicola | 番茄 | 黄化萎蔫 | |
2018-44 | Trichoderma harzianum | 辣椒 | 安全 | |
2018-45 | Aspergillus ochraceus | 番茄 | 黄化萎蔫 | |
2018-46 | Aspergillus ochraceus | 番茄 | 黄化萎蔫 | |
2018-47 | Myrothecium verrucaria | 番茄 | 干枯 | |
2018-48 | Aspergillus ochraceopetaliformis | 番茄 | 干枯 | |
2018-49 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-50 | Fusarium spp. | 番茄 | 黄化萎蔫 | |
2018-51 | Penicillium simplicissimum | 番茄 | 安全 | |
2018-52 | Metacordyceps chlamydosporia | 番茄 | 安全 | |
2018-53 | Aspergillus ochraceus | 番茄 | 干枯 | |
2018-54 | Trichoderma harzianum | 辣椒 | 安全 | |
2018-83 | Aspergillus ochraceus | 黄瓜 | 黄化萎蔫 | |
2018-84 | Aspergillus ochraceus | 黄瓜 | 干枯 | |
2018-85 | Chaetomium spp. | 黄瓜 | 黄化萎蔫 | |
2018-86 | Aspergillus ochraceus | 黄瓜 | 黄化萎蔫 | |
2018-87 | Aspergillus ochraceus | 黄瓜 | 黄化萎蔫 | |
2018-88 | Aspergillus micronesiensis | 黄瓜 | 安全 | |
2018-89 | Fusarium solani | 黄瓜 | 黄化萎蔫 | |
黑龙江 | 2018-56 | Fusarium acuminatum | 番茄 | 干枯 |
2018-58 | Fusarium solani | 茄子 | 黄化萎蔫 | |
2018-75 | Fusarium solani | 茄子 | 干枯 | |
2018-76 | Fusarium oxysporum | 辣椒 | 干枯 | |
2018-77 | Fusarium solani | 辣椒 | 黄化萎蔫 | |
2018-78 | Fusarium solani | 辣椒 | 干枯 | |
2018-82 | Fusarium oxysporum | 香瓜 | 黄化萎蔫 | |
吉林 | 2018-57 | Fusarium solani | 马铃薯 | 黄化萎蔫 |
2018-59 | Chaetomium spp. | 马铃薯 | 黄化萎蔫 | |
2018-73 | Fusarium fujikuroi | 马铃薯 | 干枯 | |
2018-74 | Fusarium solani | 马铃薯 | 安全 | |
2018-79 | Purpureocillium lilacinum | 马铃薯 | 黄化萎蔫 | |
2018-80 | Chaetomium piluliferum | 马铃薯 | 安全 | |
2018-81 | Fusarium solani | 马铃薯 | 黄化萎蔫 | |
辽宁 | 2018-55 | Simplicillium obclavatum | 黄瓜 | 黄化萎蔫 |
2018-60 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-61 | Mortierella spp. | 黄瓜 | 黄化萎蔫 | |
2018-62 | Mortierella spp. | 黄瓜 | 安全 | |
2018-63 | Fusarium oxysporum | 黄瓜 | 干枯 | |
2018-64 | Mortierella spp. | 黄瓜 | 安全 | |
2018-65 | Chaetomium piluliferum | 番茄 | 安全 | |
2018-66 | Fusarium oxysporum | 黄瓜 | 黄化萎蔫 | |
2018-67 | Fusarium oxysporum | 黄瓜 | 干枯 | |
2018-68 | Mortierella alpina | 黄瓜 | 安全 | |
2018-69 | Fusarium redolens | 黄瓜 | 黄化萎蔫 | |
2018-70 | Fusarium solani | 黄瓜 | 干枯 | |
2018-71 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-72 | Chaetomium fuscum | 番茄 | 黄化萎蔫 | |
内蒙古 | 2018-1 | Fusarium solani | 马铃薯 | 干枯 |
2018-2 | Fusarium oxysporum | 马铃薯 | 黄化萎蔫 | |
2018-3 | Fusarium solani | 马铃薯 | 干枯 | |
2018-4 | Fusarium equiseti | 马铃薯 | 安全 | |
2018-5 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-6 | Beauveria bassiana | 番茄 | 安全 | |
2018-7 | Aspergillus flavipes | 黄瓜 | 安全 | |
2018-8 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-9 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-10 | Fusarium equiseti | 番茄 | 安全 | |
2018-11 | Fusarium spp. | 黄瓜 | 黄化萎蔫 | |
2018-12 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-13 | Chaetomium piluliferum | 番茄 | 黄化萎蔫 | |
2018-14 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-15 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-16 | Fusarium solani | 黄瓜 | 黄化萎蔫 | |
2018-17 | Fusarium solani | 黄瓜 | 黄化萎蔫 | |
2018-18 | Fusarium solani | 黄瓜 | 安全 | |
2018-19 | Fusarium solani | 黄瓜 | 黄化萎蔫 | |
2018-20 | Fusarium solani | 番茄 | 黄化萎蔫 | |
2018-21 | Chaetomium piluliferum | 黄瓜 | 安全 | |
2018-22 | Fusarium cf. fujikuroi | 黄瓜 | 黄化萎蔫 | |
2018-23 | Albifimbria verrucaria | 番茄 | 安全 | |
2018-24 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-25 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-26 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-27 | Fusarium cf. equiseti | 番茄 | 黄化萎蔫 | |
2018-28 | Fusarium redolens | 番茄 | 干枯 | |
2018-29 | Fusarium oxysporum | 黄瓜 | 干枯 | |
2018-30 | Fusarium oxysporum | 番茄 | 黄化萎蔫 | |
2018-31 | Purpureocillium lilacinum | 番茄 | 黄化萎蔫 | |
2018-32 | Purpureocillium lilacinum | 番茄 | 安全 | |
2018-33 | Mortierella indohii | 番茄 | 黄化萎蔫 | |
2018-34 | Chaetomium piluliferum | 番茄 | 安全 | |
2018-35 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-36 | Fusarium equiseti | 黄瓜 | 安全 | |
2018-37 | Chaetomium piluliferum | 番茄 | 安全 | |
2018-38 | Mortierella alpina | 黄瓜 | 干枯 | |
2018-39 | Mortierella alpina | 黄瓜 | 干枯 |
试验处理 | 株高/cm | 根长/cm | 茎叶鲜重/g | 根鲜重/g | 发病率/% | 防治效果/% |
---|---|---|---|---|---|---|
32号淡紫拟青霉+FO | 20.50±0.28bc | 16.50±1.19b | 6.63±0.25c | 1.53±0.13c | 15.44±2.28a | 81.19±2.14c |
44号哈茨木霉+FO | 21.25±2.28c | 16.25±0.75b | 6.50±0.15c | 1.40±0.18c | 22.22±2.22b | 73.08±1.92b |
淡紫拟青霉菌剂+FO | 19.25±1.10bc | 15.25±1.31b | 6.33±0.21c | 1.33±0.18c | 31.11±0.11c | 61.96±0.00a |
哈茨木霉菌剂+FO | 19.50±1.25bc | 15.50±0.64b | 6.30±0.04c | 1.30±0.15c | 33.11±2.22c | 58.33±3.63a |
清水 | 16.50±0.64b | 13.50±0.95b | 5.63±0.95b | 0.85±0.11b | — | — |
FO | 9.00±1.00a | 8.00±0.81a | 3.83±0.19a | 0.35±0.65a | 82.22±2.22d | — |
试验处理 | 株高/cm | 根长/cm | 茎叶鲜重/g | 根鲜重/g | 发病率/% | 防治效果/% |
---|---|---|---|---|---|---|
32号淡紫拟青霉+FO | 20.50±0.28bc | 16.50±1.19b | 6.63±0.25c | 1.53±0.13c | 15.44±2.28a | 81.19±2.14c |
44号哈茨木霉+FO | 21.25±2.28c | 16.25±0.75b | 6.50±0.15c | 1.40±0.18c | 22.22±2.22b | 73.08±1.92b |
淡紫拟青霉菌剂+FO | 19.25±1.10bc | 15.25±1.31b | 6.33±0.21c | 1.33±0.18c | 31.11±0.11c | 61.96±0.00a |
哈茨木霉菌剂+FO | 19.50±1.25bc | 15.50±0.64b | 6.30±0.04c | 1.30±0.15c | 33.11±2.22c | 58.33±3.63a |
清水 | 16.50±0.64b | 13.50±0.95b | 5.63±0.95b | 0.85±0.11b | — | — |
FO | 9.00±1.00a | 8.00±0.81a | 3.83±0.19a | 0.35±0.65a | 82.22±2.22d | — |
试验处理 | 株高/cm | 根长/cm | 茎叶鲜重/g | 根鲜重/g | 根结指数 | 防治效果/% |
---|---|---|---|---|---|---|
32号淡紫拟青霉 | 56.25±1.75cd | 7.50±1.04b | 17.57±0.85bc | 0.53±0.14a | 8.89±0.28b | 64.06±0.73b |
44号哈茨木霉 | 51.75±0.48bc | 6.25±0.95b | 15.46±0.78bc | 0.88±0.18a | 12.22±0.28c | 50.54±1.48a |
淡紫拟青霉菌剂 | 52.25±1.11bc | 5.75±0.48b | 15.38±1.55bc | 1.00±0.28a | 9.72±0.28 b | 60.65±1.40b |
哈茨木霉菌剂 | 49.00±3.30b | 5.50±0.29b | 14.28±0.66b | 0.85±0.23a | 12.50±0.48c | 49.43±2.01a |
氟吡菌酰胺 | 60.00±2.65d | 10.75±0.85c | 20.70±3.57bc | 0.68±0.13a | 5.00±0.48a | 79.77±1.94c |
清水 | 28.75±1.71a | 2.75±0.25a | 7.20±0.98a | 2.00±0.51b | 24.72±0.28d | — |
试验处理 | 株高/cm | 根长/cm | 茎叶鲜重/g | 根鲜重/g | 根结指数 | 防治效果/% |
---|---|---|---|---|---|---|
32号淡紫拟青霉 | 56.25±1.75cd | 7.50±1.04b | 17.57±0.85bc | 0.53±0.14a | 8.89±0.28b | 64.06±0.73b |
44号哈茨木霉 | 51.75±0.48bc | 6.25±0.95b | 15.46±0.78bc | 0.88±0.18a | 12.22±0.28c | 50.54±1.48a |
淡紫拟青霉菌剂 | 52.25±1.11bc | 5.75±0.48b | 15.38±1.55bc | 1.00±0.28a | 9.72±0.28 b | 60.65±1.40b |
哈茨木霉菌剂 | 49.00±3.30b | 5.50±0.29b | 14.28±0.66b | 0.85±0.23a | 12.50±0.48c | 49.43±2.01a |
氟吡菌酰胺 | 60.00±2.65d | 10.75±0.85c | 20.70±3.57bc | 0.68±0.13a | 5.00±0.48a | 79.77±1.94c |
清水 | 28.75±1.71a | 2.75±0.25a | 7.20±0.98a | 2.00±0.51b | 24.72±0.28d | — |
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