Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 51-62.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0783

Previous Articles     Next Articles

Function of Transcription Factor CNR in the Ripening Process of Tomato Fruit

LI Ling(), YANG Li-xia, GUO Mei   

  1. College of Food Science and Biotechnology,Tianjin Agricultural University,Tianjin 300384
  • Received:2020-06-27 Online:2021-02-26 Published:2021-02-26

Abstract:

SPL transcription factors are widely existing in plants and are involved in plant growth,development and maturation. CNR is a member of SPL transcription factor family,and its mechanism of action is still unclear. The novel target genes of CNR were determined by RNA-seq,qRT-PCR and chromatin immunoprecipitation(ChIP)assay,which is aimed to uncover the transcriptional regulatory network of CNR during the ripening process of tomato fruit. About 10,223 differentially expressed genes between wild type AC and mutant cnr were achieved by RNA-seq,and these genes were involved in many aspects of tomato fruit growth and development. The results of qTR-PCR were basically consistent with transcriptome sequenced data. Particularly there were significantly large differential expression levels of gene Mannan endo-1,4-beta-mannosidase,Linoleate 9S-lipoxygenase,Pectinesterase,Phytoene synthase,1-aminocyclopropane-1-carboxylate synthase,Ethylene response factor,Polygalacturonase and Pectate lyase between AC and cnr. In addition,GTAC motif,a CNR-binding site,was found in the promoter regions of these genes,suggesting that CNR possibly directly regulated their transcriptions,and this supposition was further verified by ChIP analysis. Functional annotations of CNR target genes uncovered the specific role of CNR during the ripening process of tomato fruit via the pathways of lipid metabolism,cell wall,lycopene synthesis and ethylene biosynthesis.

Key words: CNR transcription factor, target genes, ripening process, tomato fruit, ChIP