Applications of DNA Metabarcoding in Diet Identification of Herbivores

doi:10.13560/j.cnki.biotech.bull.1985.2020-0988

Abstract

Abstract:

With the rapid development of sequencing techniques,DNA metabarcoding that derived from the combination of DNA barcoding and high-throughput sequencing technologies,has been a current research hotspot and shows great potential in diet identification of herbivores. Dietary analysis of grazing animal is a key research content in animal nutrition and grassland ecology. In contrast to traditional approach on diet determination,DNA metabarcoding can be used to investigate the species composition of a sample by high-throughput sequencing on plant DNA barcodes,and then to study the food diet of herbivores. This paper briefly reviewed the limitations of traditional diet analysis methods,and then summarized the background and analytical principles of DNA metabarcoding and its applications in the identification of herbivore diet. It also outlined the possible challenges in the research process and prospected the future trends of the DNA metabarcoding technology.

Key words: plant barcode, metabarcoding, high-throughput sequencing, herbivore, diet identification

GUO Yan-ping, ZHANG Hao, ZHAO Xin-gang, LUO Hai-ling, ZHANG Ying-jun. Applications of DNA Metabarcoding in Diet Identification of Herbivores[J]. Biotechnology Bulletin, 2021, 37(3): 252-260.

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