Effects of Ganoderma resinaceum Alcohol Extract on Sleep and Intestinal Microbiota in Mice

doi:10.13560/j.cnki.biotech.bull.1985.2021-1470

Abstract

Abstract:

The aim of this study is to investigate the effects of Ganoderma resinaceum alcohol extract（GRAE）on sleep and intestinal microbiota in mice,and to analyze the association between intestinal microbiota and sleep. The sleep rate and sleep duration of mice under sodium pentobarbital induction were observed by continuously gavaging for 30 d,and the sleep latency of mice induced by sodium barbiturate was observed. The serum of mice was tested and the effect of GRAE on the abundance of intestinal microbiota in the mice was analyzed by 16S rDNA sequencing. The results showed that GRAE prolonged the sleep duration of mice induced by sodium pentobarbital,increased the sleep rate of mice hypnotized by pentobarbital sodium subthreshold dose,shortened the sleep latency of mice induced by sodium pentobarbital,and decreased the serum levels of glucose（GLU）and triglyceride（TG）in mice. 16S rDNA results showed that GRAE increased the abundance of Bacteroidetes and Actinobacteria at the phylum level;at the genus level,GRAE increased the abundance of Bifidobacterium,and decreased the abundance of Lactobacillus and Klebsiella. Therefore,GRAE can regulate the abundance of intestinal microbiota,which in turn affects the sleep quality of mice.

Key words: Ganoderma resinaceum, alcoholic extract, sleep, intestinal microbiota, high-throughput sequencing

CHEN Tian-ci, WU Shao-lan, YANG Guo-hui, JIANG Dan-xia, JIANG Yu-ji, CHEN Bing-zhi. Effects of Ganoderma resinaceum Alcohol Extract on Sleep and Intestinal Microbiota in Mice[J]. Biotechnology Bulletin, 2022, 38(8): 225-232.

Figures/Tables 9

References 36

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组别Group	剂量Dose/（mg·kg^-1·d^-1）	0 d/g	7 d/g	14 d/g	21 d/g
CK	0	29.06±1.19	33.67±1.90	36.05±1.84	37.74±1.95
LDG	250	28.86±0.66	31.54±1.48	33.12±2.06	34.21±2.44
MDG	500	28.33±0.98	31.30±1.75	33.23±1.64	34.41±2.05
HDG	1 000	28.17±1.15	31.47±1.24	32.59±1.66	33.66±1.52

组别Group	剂量Dose/（mg·kg^-1·d^-1）	0 d/g	7 d/g	14 d/g	21 d/g
CK	0	29.06±1.19	33.67±1.90	36.05±1.84	37.74±1.95
LDG	250	28.86±0.66	31.54±1.48	33.12±2.06	34.21±2.44
MDG	500	28.33±0.98	31.30±1.75	33.23±1.64	34.41±2.05
HDG	1 000	28.17±1.15	31.47±1.24	32.59±1.66	33.66±1.52

组别 Group	巴比妥钠诱导的睡眠潜伏期 Sleep latency induced by sodium barbiturate/min	戊巴比妥钠诱导的睡眠时长 Sleep time of pentobarbital sodium-induced/min	戊巴比妥钠诱导的入睡率 Sleep rate of pentobarbital sodium-induced/%
CK	28.88±8.61a	30.94±1.54a	10
LDG	28.51±8.16a	41.87±0.63a	20
MDG	27.70±8.87a	63.23±2.32b	40
HDG	20.53±3.89b	70.12±1.88c	80

组别 Group	巴比妥钠诱导的睡眠潜伏期 Sleep latency induced by sodium barbiturate/min	戊巴比妥钠诱导的睡眠时长 Sleep time of pentobarbital sodium-induced/min	戊巴比妥钠诱导的入睡率 Sleep rate of pentobarbital sodium-induced/%
CK	28.88±8.61a	30.94±1.54a	10
LDG	28.51±8.16a	41.87±0.63a	20
MDG	27.70±8.87a	63.23±2.32b	40
HDG	20.53±3.89b	70.12±1.88c	80

Sample	Raw_Tags	Raw_Bases	Valid_Tags	Valid_Bases	Valid/%	Q20/%	Q30/%	GC/%
CK1	84 126	42.06 M	77 501	32.07 M	92.12	97.92	93.89	54.58
CK2	84 478	42.24 M	77 242	32.37 M	91.43	97.39	92.52	55.27
CK3	86 751	43.38 M	74 655	31.17 M	86.06	94.55	85.87	54.52
CK4	86 073	43.04 M	78 882	32.96 M	91.65	95.26	87.66	54.68
CK5	80 663	40.33 M	71 430	29.39 M	88.55	97.39	92.63	54.17
CK6	81 281	40.64 M	70 531	29.26 M	86.77	97.47	92.82	54.88
HDG1	84 427	42.21 M	72 261	30.28 M	85.59	97.06	91.60	54.58
HDG2	87 332	43.67 M	76 096	31.55 M	87.13	97.15	91.89	54.82
HDG3	64 752	32.38 M	52 225	21.59 M	80.65	87.32	73.18	55.95
HDG4	80 293	40.15 M	71 835	29.72 M	89.47	96.28	89.81	54.68
HDG5	86 806	43.40 M	76 963	32.22 M	88.66	97.99	93.92	55.02
HDG6	80 087	40.04 M	69 329	28.57 M	86.57	98.08	94.31	54.38