Research Status in the Production of Squalene by Thraustochytrids

doi:10.13560/j.cnki.biotech.bull.1985.2020-1148

Abstract

Abstract:

Squalene is an important polyunsaturated triterpene substance,participates in multiple physiological processes in human body,and has the effect of enhancing immunity,delaying skin aging,and preventing the cancer. Under heterotrophic conditions,Thraustochytrids can grow rapidly and synthesize high content of squalene,thus there is the potential of developing industrial production of squalene. In this paper,the application potential of ecology and biotechnology of Thraustochytrids were outlined,focusing on the research status of squalene production by Thraustochytrids,and the metabolic pathways of squalene synthesis by Thraustochytrids were summarized. Then,five main factors affecting the production of squalene in the fermentation of Thraustochytrids were reviewed,and the regulatory effects of hormone substances on the production of squalene by Thraustochytrids were summarized. The aim of this review is to provide reference for the further research and development of squalene production by Thraustochytrids.

Key words: Thraustochytrids, squalene, influencing factors, metabolic pathway, hormone

LI Meng-fan, XIE Yun-xuan, XIE Ning-dong, ZHANG Ai-qing, WANG Guang-yi. Research Status in the Production of Squalene by Thraustochytrids[J]. Biotechnology Bulletin, 2021, 37(4): 234-244.

Figures/Tables 3

References 81

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菌株 Strain	角鲨烯产量 Production of squalene	特点 Characteristics	发表时间 Published date	参考文献Reference
Aurantiochytrium sp. BR-MP4-A1	0.57 mg/g DCW	筛选鉴定出一株奥尔兰多属菌株,生物量很高,受到培养条件的高度影响	2009	[26]
Aurantiochytriummangrovei FB3	0.53 mg/g DCW	通过优化培养基和添加角鲨烯环氧化酶抑制剂（特比萘芬）来提高破囊壶菌的角鲨烯产量	2010	[3]
Aurantiochytrium sp. BR-MP4-A1	0.72 mg/g DCW	研究了不同氮源之间的相互作用对破囊壶菌的角鲨烯产量的影响	2010	[30]
Aurantiochytrium sp. 18 W-13a	198 mg/g DCW	有较高产量,可用于后续条件优化工作	2011	[31]
Schizochytrium mangrovei PQ6	80.1 mg/g DCW	从生物柴油生产过程的残渣中提取	2014	[32]
Schizochytrium sp.CCTCC 209059b	84mg/g DCW	首次报道了不皂化物（胆固醇、角鲨烯）在脂质更新阶段的增加	2014	[33]
Aurantiochytrium sp. Yonez 5-1	318 mg/g DCW	有较高产量,是采用薄层色谱法（TLC）方法筛选得到	2014	[29]
Schizochytrium limacinum B4D1	20.09 mg/g DCW	通过添加丁醇来降低DHA产量从而提高角鲨烯产量（添加6 g/L丁醇使角鲨烯含量从0.65 mg/g提高到20.09 mg/g）	2017	[34]
Aurantiochytrium sp. T66	1.0 g/L	首次使用木质纤维素作为原料来培养菌株生产角鲨烯	2019	[35]
Schizochytrium limacinum SR21	16.34 mg/g DCW	用经过化学处理的云杉处理液代替葡萄糖生产角鲨烯和DHA	2020	[36]

菌株 Strain	角鲨烯产量 Production of squalene	特点 Characteristics	发表时间 Published date	参考文献Reference
Aurantiochytrium sp. BR-MP4-A1	0.57 mg/g DCW	筛选鉴定出一株奥尔兰多属菌株,生物量很高,受到培养条件的高度影响	2009	[26]
Aurantiochytriummangrovei FB3	0.53 mg/g DCW	通过优化培养基和添加角鲨烯环氧化酶抑制剂（特比萘芬）来提高破囊壶菌的角鲨烯产量	2010	[3]
Aurantiochytrium sp. BR-MP4-A1	0.72 mg/g DCW	研究了不同氮源之间的相互作用对破囊壶菌的角鲨烯产量的影响	2010	[30]
Aurantiochytrium sp. 18 W-13a	198 mg/g DCW	有较高产量,可用于后续条件优化工作	2011	[31]
Schizochytrium mangrovei PQ6	80.1 mg/g DCW	从生物柴油生产过程的残渣中提取	2014	[32]
Schizochytrium sp.CCTCC 209059b	84mg/g DCW	首次报道了不皂化物（胆固醇、角鲨烯）在脂质更新阶段的增加	2014	[33]
Aurantiochytrium sp. Yonez 5-1	318 mg/g DCW	有较高产量,是采用薄层色谱法（TLC）方法筛选得到	2014	[29]
Schizochytrium limacinum B4D1	20.09 mg/g DCW	通过添加丁醇来降低DHA产量从而提高角鲨烯产量（添加6 g/L丁醇使角鲨烯含量从0.65 mg/g提高到20.09 mg/g）	2017	[34]
Aurantiochytrium sp. T66	1.0 g/L	首次使用木质纤维素作为原料来培养菌株生产角鲨烯	2019	[35]
Schizochytrium limacinum SR21	16.34 mg/g DCW	用经过化学处理的云杉处理液代替葡萄糖生产角鲨烯和DHA	2020	[36]

菌种 Strains	条件 Condition	角鲨烯产量 The production of squalene	发表时间 Published date	参考文献Reference
Schizochytrium mangrovei FB1	20 g/L葡萄糖	0.162 mg/g DCW	2004	[45]
Aurantiochytrium sp. BR-MP4-A1	30 g/L葡萄糖	0.72 mg/g DCW	2010	[29]
Aurantiochytrium sp. strain 18W-13a	20 g/L葡萄糖	198 mg/g DCW	2011	[31]
Aurantiochytrium sp. strain 18W-13a	20 g/L葡萄糖	171 mg/g DCW	2012	[61]
Schizochytrium mangrovei PQ6	90 g/L葡萄糖	33.04 mg/g DCW	2014	[32]
Aurantiochytrium sp. T66	通过有机溶剂预处理获得的桦木水解产物（含30 g/L葡萄糖）	69.31 mg/g DCW（烧瓶培养）88.47 mg/g DCW（生物反应器培养）	2019	[35]
Schizochytrium limacinum SR21	通过有机溶剂预处理获得的云杉水解产物（含60 g/L葡萄糖）	16.34 mg/g DCW	2020	[36]
Aurantiochytrium sp. T66	食用垃圾水解产物（含葡萄糖30 g/L,果糖9.89 g/L）	71.54 mg/g DCW	2020	[62]

菌种 Strains	条件 Condition	角鲨烯产量 The production of squalene	发表时间 Published date	参考文献Reference
Schizochytrium mangrovei FB1	20 g/L葡萄糖	0.162 mg/g DCW	2004	[45]
Aurantiochytrium sp. BR-MP4-A1	30 g/L葡萄糖	0.72 mg/g DCW	2010	[29]
Aurantiochytrium sp. strain 18W-13a	20 g/L葡萄糖	198 mg/g DCW	2011	[31]
Aurantiochytrium sp. strain 18W-13a	20 g/L葡萄糖	171 mg/g DCW	2012	[61]
Schizochytrium mangrovei PQ6	90 g/L葡萄糖	33.04 mg/g DCW	2014	[32]
Aurantiochytrium sp. T66	通过有机溶剂预处理获得的桦木水解产物（含30 g/L葡萄糖）	69.31 mg/g DCW（烧瓶培养）88.47 mg/g DCW（生物反应器培养）	2019	[35]
Schizochytrium limacinum SR21	通过有机溶剂预处理获得的云杉水解产物（含60 g/L葡萄糖）	16.34 mg/g DCW	2020	[36]
Aurantiochytrium sp. T66	食用垃圾水解产物（含葡萄糖30 g/L,果糖9.89 g/L）	71.54 mg/g DCW	2020	[62]