Temperature-related Pathogenicity Differentiation of Wheat Head Blight in Huang and Huai River Valleys and Northeast China Wheat Regions

doi:10.13560/j.cnki.biotech.bull.1985.2020-1514

Abstract

Abstract:

Pathogenicity variation in Fusarium graminearum from different locations to wheat under different temperature conditions was compared. In 2018 and 2019,diseased wheat heads were collected from the winter wheat ecological region of Huang and Huai River Valleys and spring wheat ecological region of the Northeast China,a total of 110 Fusarium strains were obtained after single-spore isolation. The EF-1α sequences of the isolated strains were used to identify the Fusarium species. The results demonstrated that F. graminearum was the predominant species causing wheat head blight in the two ecological regions. Ten F. graminearum strains from each ecological region were randomly selected and inoculation was conducted by single floret injection method in two locations of Langfang representing the high temperate region and Xining representing lower temperature region. Significant difference of pathogenicity was observed in Xining. The F. graminearum populations from the Northeast China spring wheat region showed significantly higher pathogenicity than the population from Huang and Huai River Valleys wheat region. However,the pathogenicity of the two populations was similar in Langfang site. This indicates that the F. graminearum population in the Northeast China spring wheat region has a higher adaptability at low temperature. Results of the study indicate that temperature influences the pathogenicity of F. graminearum populations,which is significant for studying the pathogenicity differentiation of F. graminearum and comprehensive control and prevention of wheat head blight.

Key words: wheat head blight, temperature, pathogenicity

LUO Li-li, ZHANG Hao, YANG Mei-xin, WANG Yun-fei, XU Jing-sheng, XU Jin, YAO Qiang, FENG Jie. Temperature-related Pathogenicity Differentiation of Wheat Head Blight in Huang and Huai River Valleys and Northeast China Wheat Regions[J]. Biotechnology Bulletin, 2021, 37(4): 47-55.

Figures/Tables 7

References 32

[1]	陆维忠. 小麦赤霉病研究[M]. 北京: 科学出版社, 2001.
	Lu WZ. Reserch on wheat head blight[M]. Beijing: Science Press, 2001.
[2]	陈云, 王建强, 杨荣明, 等. 小麦赤霉病发生危害形势及防控对策[J]. 植物保护, 2017,43(5):11-17.
	Chen Y, Wang JQ, Yang RM, et al. Current situation and management strategies of Fusarium head blight in China[J]. Plant Protection, 2017,43(5):11-17.
[3]	黄冲, 姜玉英, 吴佳文, 等, 2018年我国小麦赤霉病重发特点及原因分析[J]. 植物保护, 2019,45(2):160-163.
	Huang C, Jiang YY, Wu JW, et al. Occurrence characteristics and reason analysis of wheat head blight in 2018 in China[J]. Plant Protection, 2019,45(2):160-163.
[4]	Sutton JC. Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum[J]. Canadian Journal of Plant Pathology, 1982,4(2):195-209. doi: 10.1080/07060668209501326 URL
[5]	Pettitt TR, Parry DW, Polley RW. Effect of temperature on the incidence of nodal foot rot symptoms in winter wheat crops in England and Wales caused by Fusarium culmorum and Microdochium nivale[J]. Agricultural and Forest Meteorology, 1996,79(4):233-242. doi: 10.1016/0168-1923(95)02281-3 URL
[6]	Zhou F. Review of compendium of wheat diseases and pests[J]. Journal of Agricultural & Food Information, 2011,12(2):210.
[7]	Vigier B, Reid LM, Seifert KA, et al. Distribution and prediction of Fusarium species associated with maize ear rot in Ontario[J]. Canadian Journal of Plant Pathology, 1997,19(1):60-65. doi: 10.1080/07060669709500574 URL
[8]	Parry DW, Jenkinson P, Mcleod L. Fusarium ear blight(scab)in small grain cereals-a review[J]. Plant Pathology, 1995,44:207-238. doi: 10.1111/ppa.1995.44.issue-2 URL
[9]	Daamen RA, Langerak CJ, Stol W. Surveys of cereal diseases and pests in the Netherlands. 3. Monographella nivalis and Fusarium spp. in winter wheat fields and seed lots[J]. Netherlands Journal of Plant Pathology, 1991,97(2):105-114. doi: 10.1007/BF01974274 URL
[10]	徐雍皋, 方中达. 玉蜀黍赤霉对小麦品种致病力的测定方法和致病力的分化[J]. 植物病理学报, 1982,12(4):55-59.
	Xu YG, Fang ZD. Methods of testing the resistance of wheat varieties to the scab and the differentiation of the virulence of the causal organism[J]. Acta Phytopathologica Sinica, 1982,12(4):55-59.
[11]	Bai GH, Desjardins AE, Plattner RD. Deoxynivalenol-nonproducing Fusarium graminearum causes initial infection, but does not cause disease spread in wheat spikes[J]. Mycopathologia, 2002,153(2):91-98. pmid: 12000132
[12]	赵纯森, 马星霞, 武爱波, 等. 禾谷镰刀菌培养性状与致病力的相关性分析[J]. 华中农业大学学报, 2005,24(3):254-257.
	Zhao CS, Ma XX, Wu AB, et al. Correlation analysis between culture phenotypes of Fusarium graminearum schwabe isolates and their pathogenicity[J]. Journal of Huazhong Agricultural, 2005,24(3):254-257.
[13]	Carter JP, Rezanoor HN, Holden D, et al. Variation in pathogenicity associated with the genetic diversity of Fusarium graminearum[J]. European Journal of Plant Pathology, 2002,108(6):573-583. doi: 10.1023/A:1019921203161 URL
[14]	理永霞, 张星耀. 我国中温带面临的松材线虫入侵扩张高风险[J]. 温带林业研究, 2018,1(1):3-6.
	Li YX, Zhang XY. High risk of invasion and expansion of pine wood nematode in middle temperate zone of China[J]. Journal of Temperate Forestry Research, 2018,1(1):3-6.
[15]	盛若成, 李敏, 陈军, 等. 两株我国南北松材线虫虫株形态指标与致病力比较[J]. 南京林业大学学报:自然科学版, 2019,43(6):18-24.
	Sheng RC, Li M, Chen J, et al. Comparison of morphological index and pathogenicity of two isolates of Bursaphelenchus xylophilus in southern and northern in China[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2019,43(6):18-24.
[16]	Lannou C. Variation and selection of quantitative traits in plant pathogens[J]. Annual Review of Phytopathology, 2012,50(1):319-338. doi: 10.1146/annurev-phyto-081211-173031 URL
[17]	Ellison CE, Hall C, Kowbel D, et al. Population genomics and local adaptation in wild isolates of a model microbial eukaryote[J]. Proceedings of the National Academy of Sciences of the United States of America, 2011,108(7):2831-2836.
[18]	张昊, 张争, 许景升, 等. 一种简单快速的赤霉病菌单孢分离方法——平板稀释画线分离法[J]. 植物保护, 2008,34(6):134-136.
	Zhang H, Zhang Z, Xu JS, et al. A rapid and simple method for obtaining single-spore isolates of Fusarium species-agar dilution lineation separation[J]. Plant Protection, 2008,34(6):134-136.
[19]	潘晓静, 陈楠, 姚远, 等. 东北地区小麦赤霉病镰孢菌种群及其致病性测定[J]. 华北农学报, 2015,30(3):205-210.
	Pan XJ, Chen N, Yao Y, et al. Population and pathogenicity of Fusarium spp. causing wheat head blight in northeast of China[J]. Acta Agriculturae Boreali-Sinica, 2015,30(3):205-210.
[20]	Katoh K, Kuma KI, Toh H, et al. MAFFT version 5:improvement in accuracy of multiple sequence alignment[J]. Nucleic Acids Research, 2005,33(2):511-518. doi: 10.1093/nar/gki198 URL
[21]	Nguyen LT, Schmidt HA, von Haeseler A, et al. IQ-TREE:a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies[J]. Molecular Biology and Evolution, 2015,32(1):268-274. doi: 10.1093/molbev/msu300 URL
[22]	Wang LG, Lam TTY, Xu SB, et al. Treeio:an R package for phylogenetic tree input and output with richly annotated and associated data[J]. Molecular Biology and Evolution, 2020,37(2):599-603. doi: 10.1093/molbev/msz240 URL
[23]	Yu GC, Smith DK, Zhu HC, et al. Ggtree:an r package for visualization and annotation of phylogenetic trees with their covariates and other associated data[J]. Methods in Ecology and Evolution, 2017,8(1):28-36. doi: 10.1111/mee3.2017.8.issue-1 URL
[24]	Wei SH, van der Lee T, Verstappen E, et al. Targeting trichothecene biosynthetic genes[J]. Methods in Molecular Biology, 2017,1542:173-189.
[25]	徐飞, 杨共强, 王俊美, 等. 河南省小麦赤霉病菌种群组成及致病力分化[J]. 植物病理学报, 2016,46(3):294-303.
	Xu F, Yang GQ, Wang JM, et al. Composition and variation in aggressiveness of Fusarium populations causing wheat head blight in Henan Province[J]. Acta Phytopathologica Sinica, 2016,46(3):294-303.
[26]	Zhang H, van der Lee T, Waalwijk C, et al. Population analysis of the Fusarium graminearum species complex from wheat in China show a shift to more aggressive isolates[J]. PLoS One, 2012,7(2):e31722. doi: 10.1371/journal.pone.0031722 URL
[27]	Zhang YJ, Yu JJ, Zhang YN, et al. Effect of carbendazim resistance on trichothecene production and aggressiveness of Fusarium graminearum[J]. Molecular Plant-Microbe Interactions, 2009,22(9):1143-1150. doi: 10.1094/MPMI-22-9-1143 URL
[28]	纪莉景, 栗秋生, 王亚娇, 等. 温度对假禾谷镰刀菌生长、侵染及茎基腐病发生的影响[J]. 植物病理学报, 2020,50(6):723-730.
	Ji LJ, Li QS, Wang YJ, et al. The effect of temperature on the growth and infection of Fusarium pseudograminearum and the occurrence of stem base rot[J]. Acta Phytopath Ologia Sinica, 2020,50(6):723-730.
[29]	Maurin N, Saur L, Capron G. Stem and head reaction of winter wheat cultivars to artificial inoculation by Microdochium nivale under controlled environment and field conditions[J]. Euphytica, 1995,92(3):359-366. doi: 10.1007/BF00037120 URL
[30]	Brennan JM, Fagan B, van Maanen A, et al. Studies on in vitro growth and pathogenicity of European Fusarium fungi[J]. European Journal of Plant Pathology, 2003,109(6):577-587. doi: 10.1023/A:1024712415326 URL
[31]	Wang DH, Barbetti MJ, Sivasithamparam K. Effects of temperature and moisture on the pathogenicity of fungi associated with root rot of subterranean clover[J]. Australian Journal of Agricultural Research, 1984,35:675-684. doi: 10.1071/AR9840675 URL
[32]	Sabburg R, Obanor F, Aitken E. Effect on increasing temperature on the pathogenic fitness of Fusarium pseudograminearum[J]. Procedia Environmental Sciences, 2015,29:182-183. doi: 10.1016/j.proenv.2015.07.250 URL

小麦种植区 Wheat growing area	采样点 Sampling site	经度Longitude	纬度 Altitude	2018年分离菌株数量 Number of isolates in 2018	2019年分离菌株数量Number of isolates in 2019	总计 Total
黄淮冬麦区 Huanghuai winter wheat region	河南	114.192389	35.05454097	1（1）	1（1）	2
	Henan	114.478073	35.02427601	1	0	1
		115.197768	36.039024	2（1）	1（1）	3
		113.596423	35.08097104	0	3	3
		114.003443	35.043789	1	3	4
		114.56618	34.83769602	1	1	2
		115.150602	35.47239896	1	0	1
	河北	115.824965	39.218749	3（1）	5（1）	8
	Hebei	115.227049	38.66614404	3（1）	4（1）	7
		115.04654	38.52423398	2	0	2
		114.550125	36.75152097	10（1）	6（1）	16
		114.614042	36.605715	3	6	9
		115.119011	36.27501296	0	1	1
		115.191918	41.248323	2	0	2
东北春麦区Northeast spring wheat region	内蒙古	120.195951	50.64350003	0	1	1
	Inner Mongolia	119.705403	49.53917599	3（1）	4（1）	7
		119.775435	49.64931303	5	0	5
		120.111686	49.41728602	5（1）	6（1）	11
		120.675101	49.375361	0	1	1
		120.804145	49.298681	2（1）	3（1）	5
		120.932706	49.31512898	1（1）	4（1）	5
		120.446777	49.54273101	7（1）	5（1）	12
		120.472253	49.47417199	1	0	1
		120.675101	49.375361	0	1	1
总计total				54	56	110

小麦种植区 Wheat growing area	采样点 Sampling site	经度Longitude	纬度 Altitude	2018年分离菌株数量 Number of isolates in 2018	2019年分离菌株数量Number of isolates in 2019	总计 Total
黄淮冬麦区 Huanghuai winter wheat region	河南	114.192389	35.05454097	1（1）	1（1）	2
	Henan	114.478073	35.02427601	1	0	1
		115.197768	36.039024	2（1）	1（1）	3
		113.596423	35.08097104	0	3	3
		114.003443	35.043789	1	3	4
		114.56618	34.83769602	1	1	2
		115.150602	35.47239896	1	0	1
	河北	115.824965	39.218749	3（1）	5（1）	8
	Hebei	115.227049	38.66614404	3（1）	4（1）	7
		115.04654	38.52423398	2	0	2
		114.550125	36.75152097	10（1）	6（1）	16
		114.614042	36.605715	3	6	9
		115.119011	36.27501296	0	1	1
		115.191918	41.248323	2	0	2
东北春麦区Northeast spring wheat region	内蒙古	120.195951	50.64350003	0	1	1
	Inner Mongolia	119.705403	49.53917599	3（1）	4（1）	7
		119.775435	49.64931303	5	0	5
		120.111686	49.41728602	5（1）	6（1）	11
		120.675101	49.375361	0	1	1
		120.804145	49.298681	2（1）	3（1）	5
		120.932706	49.31512898	1（1）	4（1）	5
		120.446777	49.54273101	7（1）	5（1）	12
		120.472253	49.47417199	1	0	1
		120.675101	49.375361	0	1	1
总计total				54	56	110

地区 Area	廊坊 Langfang	西宁 Xining
黄淮 Huanghuai	0.202 ± 0.124	0.359 ± 0.153
东北 Northeast China	0.214 ± 0.177	0.254 ± 0.097

地区 Area	廊坊 Langfang	西宁 Xining
黄淮 Huanghuai	0.202 ± 0.124	0.359 ± 0.153
东北 Northeast China	0.214 ± 0.177	0.254 ± 0.097

群体 Group	自由度 Df	偏差 Deviance	残差自由度 Resid. Df	残差偏差 Resid. Dev	P值 P（>Chi）
廊坊群体 Langfang	1	0.64626	271	1193.7	0.7001
西宁群体 Xining	1	42.995	2998	334.86	1.359×10^-10