Fig.1 Relative expressions of ZmJAZ4,ZmJAZ8,and ZmJAZ12 En：Endosperm. Em：Embryo. DAP：Day after pollination. The data are in means ± SD

Fig.2 Vectors for subcellular localization and enzyme digestion identification A：Schematic diagram of vectors for subcellular localization,including control GFP vector and cell nucleus localization marker vector. B：Enzyme digestion identification of Xho I + Xba I in subcellular localization vectors. M1：100 bp DNA ladder. M2：1000 bp DNA ladder. Lane 1：pZmMYC2-GFP,lane 2：pZmJAZ4-GFP,lane 3：pZmJAZ8-GFP,and lane 4：pZmJAZ12-GFP

Fig.3 Vectors for BiFC assay and enzyme digestion identification A：Schematic diagram of vectors for BiFC assay. B：Enzyme digestion identification of BiFC vectors by Xho I + Xba I. M1：100 bp DNA ladder. M2：1 000 bp DNA ladder. Lane 1：pZmMYC2-YFPN,lane 2：pZmMYC2-YFPC,lane 3：pZmJAZ4-YFPN,lane 4：pZmJAZ4-YFPC,lane 5：pZmJAZ8-YFPN,lane 6：pZmJAZ8-YFPC,lane 7：pZmJAZ12-YFPN,and lane 8：pZmJAZ12-YFPC

Fig. 4 Subcellular localization of ZmJAZ4,ZmJAZ8,ZmJAZ12 and ZmMYC2 The ORFs of ZmJAZ4、ZmJAZ8、ZmJAZ12 and ZmMYC2 were fused at the N terminal of GFP. GFP indicates the target protein fluorescence channel. DsRed indicates fluorescence channel for a nuclear localization maker. Chl indicates the chloroplast spontaneous fluorescence channel. Bright indicates the bright field. The Merge indicates channel combined the GFP,DsRed,Chl and the bright field. Scale bar = 10 μm

Fig. 5 BiFC assay to verify interactions between ZmJAZs with ZmMYC2 ZmMYC2 and 3 to-be-verified genes were constructed into the N-terminal and C-terminal of YFP for BiFC assay. YFP indicates fluorescence channel triggered by BiFC interaction. The DsRed indicates fluorescence channel for nuclear localization maker. Chl indicates the chloroplast spontaneous fluorescence channel. Bright indicates the bright field. Merge indicates channel combined the GFP,DsRed,Chl and the bright field. Scale bar = 10 μm