Screening of Potential PGPR Strains Producting Growth-promoting Volatile Compounds and Study on Their Growth-promoting Characteristics

doi:10.13560/j.cnki.biotech.bull.1985.2021-0619

Abstract

Abstract:

The aim of this work is to screen the potential plant growth-promoting rhizobacteria（PGPR）whose volatile organic compounds（VOCs）can promote plant growth,and to investigate the growth-promoting effect of the VOCs on plants and other growth-promoting functions,which provides new ideas and reliable materials for the research and development of microbial fertilizers. Using 48 functional bacteria isolated from marine samples and preserved in our laboratory as test strains,two-compartment plate test and VOCs pot experiment were used to screen them,16S rRNA to identify them,and GC-MS to analyze the VOCs components produced by the strains. Finally plate activity test was applied to detect the activities of the bacteria for nitrogen fixation,phosphorus solubilization and IAA production. The results showed that a strain of potential PGPR GX14001 was obtained. The VOCs produced by PGPR-GX14001 showed obvious growth-promoting effects on both Nicotiana benthamiana and Brassica chinensis L. seedlings. The strain GX14001 was identified as Microbacterium aurantiacum by 16S rRNA. Seven specific compounds were obtained by GC-MS analysis of VOCs. The GX14001 had strong dissolved organic/inorganic phosphorus activity and general nitrogen fixation activity,but weak IAA production capacity,which was 1.737 μg/mL. PGPR promoted the growth of plants through different growth-promoting mechanisms. The results showed that the VOCs produced by GX14001 had obvious growth-promoting effect on plants,but not high in the other aspects of growth-promoting activity;and there was no high positive correlation between them,indicating that the most important growth-promoting effect was via the produced VOCs.

Key words: plant growth-promoting rhizobacteria（PGPR）, volatile organic compounds（VOCs）, Microbacterium aurantiacum, phosphorus solubilization

GAO Ya-hui, JIANG Ming-guo, FENG Jing, ZHOU Gui. Screening of Potential PGPR Strains Producting Growth-promoting Volatile Compounds and Study on Their Growth-promoting Characteristics[J]. Biotechnology Bulletin, 2022, 38(3): 103-112.

Figures/Tables 9

References 32

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菌株编号Strain No.	菌株名称Name of strain
GX13159	Bacillus cereus
GX13529	Staphylococcus edaphicus
GX13571	Asticcacaulis excentricus
GX13580	Micrococcus aloeverae
GX13585	Microbacterium hydrothermale
GX13594	Microbacterium laevaniformans
GX13656	Bacillus acidiceler
GX13667	Bacillus aryabhattai
GX13668	Micrococcus aloeverae
GX13676	Streptomyces diastaticus subsp. ardesiacus
GX13678	Streptomyces sanglieri
GX13679	Falsibacillus pallidus
GX13685	Sinomonas humi
GX13699	Cellulosimicrobium funkei
GX13717	Kocuria indica
GX13747	Paenibacillus silvae
GX13767	Bacillus mangrovi
GX13774	Bacillus marisflavi
GX13777	Halobacillus marinus
GX13785	Kosakonia oryziphila
GX13790	Serratia oryzae
GX14001	Microbacterium aurantiacum
GX14008	Microbacterium maritypicum
GX14011	Exiguobacterium aestuarii
GX14016	Staphylococcus haemolyticus
GX14017	Staphylococcus hominis subsp. novobiosepticus
GX14022	Staphylococcus nepalensis
GX14028	Kytococcus sedentarius
GX14031	Sporosarcina koreensis
GX14032	Macrococcus canis
GX14046	Glutamicibacter creatinolyticus
GX14059	Pontibacter chinhatensis
GX14066	Kocuria tytonicola
GX14070	Micrococcus luteus
GX14080	Macrococcus caseolyticus subsp. Caseolyticus
GX14105	Bacillus altitudinis
GX14108	Brachybacterium squillarum
GX14201	Staphylococcus sciuri
GX14203	Bacillus haikouensis
GX14062	Agromyces indicus
GX14082	Psychrobacter pulmonis
GX14096	Pseudomonas deceptionensis
GX13112	Klebsiella Trevisan
GX13213	Klebsiella variicola subsp. Variicola
GX14104	Streptomyces griseus subsp. griseus
GX14111	Streptomyces koyangensis
GX14119	Streptomyces gramineus
GX14125	Streptomyces flavovirens

菌株编号Strain No.	菌株名称Name of strain
GX13159	Bacillus cereus
GX13529	Staphylococcus edaphicus
GX13571	Asticcacaulis excentricus
GX13580	Micrococcus aloeverae
GX13585	Microbacterium hydrothermale
GX13594	Microbacterium laevaniformans
GX13656	Bacillus acidiceler
GX13667	Bacillus aryabhattai
GX13668	Micrococcus aloeverae
GX13676	Streptomyces diastaticus subsp. ardesiacus
GX13678	Streptomyces sanglieri
GX13679	Falsibacillus pallidus
GX13685	Sinomonas humi
GX13699	Cellulosimicrobium funkei
GX13717	Kocuria indica
GX13747	Paenibacillus silvae
GX13767	Bacillus mangrovi
GX13774	Bacillus marisflavi
GX13777	Halobacillus marinus
GX13785	Kosakonia oryziphila
GX13790	Serratia oryzae
GX14001	Microbacterium aurantiacum
GX14008	Microbacterium maritypicum
GX14011	Exiguobacterium aestuarii
GX14016	Staphylococcus haemolyticus
GX14017	Staphylococcus hominis subsp. novobiosepticus
GX14022	Staphylococcus nepalensis
GX14028	Kytococcus sedentarius
GX14031	Sporosarcina koreensis
GX14032	Macrococcus canis
GX14046	Glutamicibacter creatinolyticus
GX14059	Pontibacter chinhatensis
GX14066	Kocuria tytonicola
GX14070	Micrococcus luteus
GX14080	Macrococcus caseolyticus subsp. Caseolyticus
GX14105	Bacillus altitudinis
GX14108	Brachybacterium squillarum
GX14201	Staphylococcus sciuri
GX14203	Bacillus haikouensis
GX14062	Agromyces indicus
GX14082	Psychrobacter pulmonis
GX14096	Pseudomonas deceptionensis
GX13112	Klebsiella Trevisan
GX13213	Klebsiella variicola subsp. Variicola
GX14104	Streptomyces griseus subsp. griseus
GX14111	Streptomyces koyangensis
GX14119	Streptomyces gramineus
GX14125	Streptomyces flavovirens

组别 Group	鲜重 Fresh weight/g	根长 Root lengt/cm	侧根数 Number of lateral root/roots	叶片长 Leaf length/cm	叶片宽 Leaf width/cm
对照组Control	0.081±0.015	2.855±0.656	1.887±0.726	0.551±0.093	0.443±0.089
GX13594	0.503±0.214^**	4.627±1.481^**	4.400±1.183^**	0.990±0.217^**	0.730±0.158^**
GX13747	0.387±0.078^**	5.187±1.351^**	4.267±0.799^**	1.023±0.145^**	0.772±0.157^**
GX14001	0.703±0.300^**	4.530±1.129^**	4.267±1.870^**	1.250±0.260^**	0.880±0.190^**
GX14016	0.487±0.106^**	4.853±1.255^**	5.333±1.589^**	1.133±0.247^**	0.775±0.191^**
GX14066	0.620±0.100^**	4.243±0.918^**	4.533±1.552^**	1.285±0.244^**	0.958±0.260^**
GX14070	0.300±0.183^**	4.600±0.858^**	3.867±1.125^**	0.950±0.163^**	0.742±0.129^**
GX14201	0.457±0.093^**	4.486±1.143^**	4.643±1.082^**	1.107±0.289^**	0.764±0.209^**

组别 Group	鲜重 Fresh weight/g	根长 Root lengt/cm	侧根数 Number of lateral root/roots	叶片长 Leaf length/cm	叶片宽 Leaf width/cm
对照组Control	0.081±0.015	2.855±0.656	1.887±0.726	0.551±0.093	0.443±0.089
GX13594	0.503±0.214^**	4.627±1.481^**	4.400±1.183^**	0.990±0.217^**	0.730±0.158^**
GX13747	0.387±0.078^**	5.187±1.351^**	4.267±0.799^**	1.023±0.145^**	0.772±0.157^**
GX14001	0.703±0.300^**	4.530±1.129^**	4.267±1.870^**	1.250±0.260^**	0.880±0.190^**
GX14016	0.487±0.106^**	4.853±1.255^**	5.333±1.589^**	1.133±0.247^**	0.775±0.191^**
GX14066	0.620±0.100^**	4.243±0.918^**	4.533±1.552^**	1.285±0.244^**	0.958±0.260^**
GX14070	0.300±0.183^**	4.600±0.858^**	3.867±1.125^**	0.950±0.163^**	0.742±0.129^**
GX14201	0.457±0.093^**	4.486±1.143^**	4.643±1.082^**	1.107±0.289^**	0.764±0.209^**

编号No.	保留时间Retaining time/ min	化学名称Chemical name	峰面积Peak area
1	9.3353	邻乙基甲苯（1-ethyl-2-methyl- Benzene）	1335701
2	9.9132	1,2,3-三甲苯（1,2,3-trimethyl- Benzene）	2357671
3	11.1091	1,2,4-三甲苯（1,2,4-trimethyl- Benzene）	294469
4	11.298	4-甲基-癸烷（4-methyl-Decane）	917363
5	12.8658	十二烷（Dodecane）	3497194
6	32.2232	二十五烷（Pentacosane）	671059
7	34.1057	9-辛基-十七烷（9-octyl-Heptadecane）	387135