The aims are to explore the laccase-producing capacities of different Lentinula edodes strains in Guizhou and obtain a high-yield laccase cultivation substrate,and therefore lay a theoretical foundation for increasing and improving the yield and quality of L. edodes,for achieving the purpose of revitalizing the mushroom industry in Guizhou. Taking Guizhou L. edodes 808,Qingke R20 and Guizhou’s unique Masang L. edodes as the research objects,the discriminative identification of the three species of L. edodes strains was preliminarily studied through antagonistic reaction,phylogenetic tree and temperature tests. And the main cultivation substrates of L. edodes were used as an inducer,the changing pattern on the laccase production activities of three different L. edodes strains in liquid fermentation for 12 consecutive days were studied. Although Masang L. edodes,L. edodes 808 and Qingke R20 were all subordinate branches of organic L. edodes,and there were differences among the strains,meaning there were certain provenance differences. The liquid laccase activity of L. edodes was significantly affected by the genetic differences of the strains and the 4 different induction media（P<0.001）,and the laccase activity showed a trend of increasing first and then decreasing under each treatment. The laccase activity of L. edodes was lower under all treatments in the early stage of culture,higher in the middle and late stages of culture,and the peak value was mainly concentrated in the 8-11 d. Compared with the strain Qingke R20 and Masang L. edodes,L. edodes 808 had the strongest liquid laccase-producing capacity in the induction medium containing corn cob,Coriariane palensis sawdust and Quercus acutissima sawdust,and their maximum enzyme activity was 464.06,238.64 and 288.94 U/L,respectively. However,wheat bran was more inducible to Qingke R20,with the maximum enzyme activity reaching 265.07 U/L. In addition,wheat bran presented the most concentrated and rapid response time for inducing laccase production from Masang L. edodes,with the maximum enzyme activity reaching 161.83 U/L. However,the high level of inducibility was more durable in C. palensis sawdust,with the maximum enzyme activity reaching 108.61 U/L. In conclusion,there are differences among the three strains of Masang L. edodes,L. edodes 808 and Qingke R20,meaning that there were certain provenance differences. From the perspective of laccase production level,L. edodes 808,Qingke R20 and Masang L. edodes had great differences in lignin degradation and absorption capacity of 4 substrates,and their best substrates were corn cob,wheat bran and C. palensis sawdust,respectively.
