QTL Mapping for Resistance to Rice Kernel Smut of Male Sterile Line

doi:10.13560/j.cnki.biotech.bull.1985.2021-0934

Abstract

Abstract:

In order to locate the resistance gene of rice kernel smut and establish the molecular breeding system for resistance to rice kernel smut,a RIL（recombinant inbred line）population consisting of 183 high-generation stable male sterile lines was constructed by hybridization and multi-year self-crossing using W9593S,a PTGMS（photo- and thermo-sensitive genic male sterile）line that has strong resistance to rice kernel smut,and Pei'ai 64S,a PTGMS line that has the same sterile allele of W9593S and is susceptible to rice kernel smut,as parent lines. With the RIL population as material,the QTL for resistance to rice kernel smut was mapped by composite interval mapping using 200 polymorphic SNP（single nucleotide polymorphism）molecular markers. The result showed that,5 QTLs with resistance to rice kernel smut were located on 4 chromosomes with phenotypic contribution rates ranging from 7.26% to 13.64%. Among them,qRRKS-11 and qRRKS-8 had the highest phenotypic contribution rates of 13.64% and 12.07%,respectively,and the genetic distances of qRRKS-11 and qRRKS-8 were 0.9 and 1.4 cM,respectively. The resistance to kernel smut is characterized by quantitative trait inheritance in rice,and the molecular marker-assisted breeding system of rice sterile lines resistant to kernel smut may be achieved by fine mapping and cloning of major QTL.

Key words: rice（Oryza sativa）, male sterile line, resistance to rice kernel smut, QTL mapping

YANG Yi-shan, SUN Ping-yong, YU Mu-lan. QTL Mapping for Resistance to Rice Kernel Smut of Male Sterile Line[J]. Biotechnology Bulletin, 2022, 38(3): 16-21.

Figures/Tables 4

Fig.1 Frequency distribution of rice kernel smut rate in RIL population

Fig. 2 Chromosome distribution of differential SNP marker between parents W9593S and Pei'ai 64 S The green indicates the rice chromosome and the blue line indicates the differential SNP marker,and the number on the top of the chromosome refers to the number of markers

Fig. 3 Chromosome distribution of QTL for resistance to rice kernel smut

Table 1 Mapping results of QTL for resistance to rice kernel smut

QTL	染色体 Chromosome	连锁标记区间 Linkage marker interval	遗传距离 Genetic distance/cM	加性效应^* Additive effect	表型贡献率 Phenotypic contribution rate/%	LOD值LOD value
qRRKS-1.1	1	K_010401-K_010402	20.5	-7.04	10.89	3.08
qRRKS-1.2	1	K_010403-K_010365	1.6	-6.17	8.03	3.29
qRRKS-4	4	K_040249-K_040266	34.8	-5.75	7.26	2.51
qRRKS-8	8	K_080131-K_080138	1.4	-7.41	12.07	5.13
qRRKS-11	11	K_110403-K_110016	0.9	7.87	13.64	5.82

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