Response of Chalcone Synthase Gene（CHS）to Different Light Quality and Transcription Factor Regulation in Vitis davidii

doi:10.13560/j.cnki.biotech.bull.1985.2022-0077

Abstract

Abstract:

Chalcone synthase（CHS）is the first key enzyme in anthocyanidin synthesis pathway. To explore the CHS expression pattern under different light quality and transcription factor regulation，2 CHS genes（VdCHS2 and VdCHS3）were cloned from Vitis davidii callus，and VdCHS bioinformatics and relative expression by 8 different light quality treatments were conducted. Moreover，CHS promoter cis-acting elements and transcription factor binding sites were predicted. The results showed that VdCHS2 and VdCHS3 were cloned with 1 382 bp and 1 398 bp length，respectively. Both CHS were composed of 2 exons and 1 intron，and they encoded hydrophilic proteins without signal peptide，and were predicted to be cytoplasm-located. Light promoted the expression of VdCHS，which was up-regulated under short wavelength light induction，then down-regulated when its expression level reached a certain level. But long wavelength light inhibited the expression of VdCHS. Total 13 MYB transcription factors targeted at CHS were predicted，and there were 23 MYB recognition and binding elements on the 2 CHS promoters. The above results revealed that light quality affected greatly the expression of CHS. Moreover，13 MYB transcription factors may be involved in CHS transcription regulation in anthocyanidin synthesis of V. davidii.

Key words: Vitis davidii, chalcone synthase, CHS, light quality, transcription factor, MYB, anthocyanidin

LAI Gong-ti, QUE Qiu-xia, PAN Ruo, LIU Yu-xuan, WANG Qi, LAI Pu-fu, GAO Hui-ying, LAI Cheng-chun. Response of Chalcone Synthase Gene（CHS）to Different Light Quality and Transcription Factor Regulation in Vitis davidii[J]. Biotechnology Bulletin, 2022, 38(11): 129-139.

Figures/Tables 10

References 52

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引物名称 Primer name	引物序列 Primer sequence（5'-3'）	用途 Application
CHS2-F	CACTGATTCAAGCAGCCAAAAATG	CHS2 克隆
CHS2-R	TTACTTAAGTGTGAAAGGAGAGAAG	CHS2 克隆
CHS3-F	CTCCAACCACCACAACCCTCATC	CHS3 克隆
CHS3-R	ATGGGGCTAGAAAAAGCATATCATC	CHS3 克隆
CHS2-qPCR-F	TGGGTCTGAAGGAAGAGAAAC	CHS2 qPCR
CHS2-qPCR-R	TTGTGTAGCAAGGCTGTGC	CHS2 qPCR
CHS3-qPCR-F	AGTTACGCTCCACACGACAC	CHS3 qPCR
CHS3-qPCR-R	AGCACCACGGTCTCAACAGT	CHS3 qPCR

引物名称 Primer name	引物序列 Primer sequence（5'-3'）	用途 Application
CHS2-F	CACTGATTCAAGCAGCCAAAAATG	CHS2 克隆
CHS2-R	TTACTTAAGTGTGAAAGGAGAGAAG	CHS2 克隆
CHS3-F	CTCCAACCACCACAACCCTCATC	CHS3 克隆
CHS3-R	ATGGGGCTAGAAAAAGCATATCATC	CHS3 克隆
CHS2-qPCR-F	TGGGTCTGAAGGAAGAGAAAC	CHS2 qPCR
CHS2-qPCR-R	TTGTGTAGCAAGGCTGTGC	CHS2 qPCR
CHS3-qPCR-F	AGTTACGCTCCACACGACAC	CHS3 qPCR
CHS3-qPCR-R	AGCACCACGGTCTCAACAGT	CHS3 qPCR

基因名称 Gene name	GenBank 登陆号 GenBank accession	片段长度 Fragment length/bp	ORF长度 ORF length/bp	氨基酸数目 Amino acid number	分子量 Molecular weight/kD	理论等电点pI	亲水性 GRAVY	信号肽 Signal peptide	亚细胞定位 Subcellular localization
VdCHS2	OL906400	1 382	1 182	393	42.90	6.1	-0.057	无 No	细胞质Cytoplasmic
VdCHS3	OL906401	1 398	1 170	389	42.60	6.18	-0.132	无 No	细胞质Cytoplasmic

基因名称 Gene name	GenBank 登陆号 GenBank accession	片段长度 Fragment length/bp	ORF长度 ORF length/bp	氨基酸数目 Amino acid number	分子量 Molecular weight/kD	理论等电点pI	亲水性 GRAVY	信号肽 Signal peptide	亚细胞定位 Subcellular localization
VdCHS2	OL906400	1 382	1 182	393	42.90	6.1	-0.057	无 No	细胞质Cytoplasmic
VdCHS3	OL906401	1 398	1 170	389	42.60	6.18	-0.132	无 No	细胞质Cytoplasmic

光质类型 Light quality type	光质名称 Light quality	光波长 Light wavelength/nm		表达模式 Expression pattern
光质类型 Light quality type	光质名称 Light quality	主峰 Main peak	次峰 Secondary peak	VdCHS2	VdCHS3
短波光	紫光	448	/	先升后降	先升后降
	蓝光	456	/	先升后降	先升后降
	绿光	514	/	先升后降	先升后降
长波光	黄光	590	/	下调	下调
长波光	红光	637	/	下调	下调
混合光质	白光	453	550	先升后降	下调
	暖白光	588	450	先升后降	上调
	暖黄光	595	450	先升后降	先升后降