Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (11): 140-150.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0091

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Analysis of Morphological Characteristics and Genetic Variation in a New Germplasm Lilium lancifolium JD-h-15

FU Yong-yao1(), YI De-yan1, YANG Xian-mao1, CAI Li1, LIANG Yu-hua1, LEI Mei-yan2(), YANG Li-ping1()   

  1. 1. School of Advanced Agriculture and Bioengineering,Yangtze Normal University,Chongqing 408100
    2. Chongqing Institute of Medicinal Plant Cultivation,Chongqing 408435
  • Received:2022-01-19 Online:2022-11-26 Published:2022-12-01
  • Contact: LEI Mei-yan,YANG Li-ping E-mail:34331107@qq.com;leimeiyan1985@126.com;yangliping1962@126.com

Abstract:

In order to cultivate a high-quality new germplasm of Lilium lancifolium,JD-h-15 induced by colchicine was used to analyze the chromosome ploidy after 3 years’ planting in the field. The plant morphology,stigma and pollen fertility,leaf epidermis and starch grain characteristics,and active components of bulb were compared in JD-h-15 and L. lancifolium control. Further their difference on molecular levels was analyzed by ISSR marker technology. The results showed that JD-h-15 has restored to be a triploid plant from an aneuploid chimera before. The plant height,leaf interval and leaf area of JD-h-15 significantly increased compared with that in the control,as well as the stem diameter and leaf number per plant decreased,in addition the flowering period per plant was advanced in JD-h-15 by 14 d. The single head rate of bulb in JD-h-15 was 93.75%,significantly higher than 70.00% of that in the control. Analysis of stigma acceptability in different flower periods revealed the stigma in 50-60 mm flower bud of JD-h-15 had a higher acceptability than that in the control. 13.71% of the pollen for JD-h-15 germinated normally,while the control pollen did not germinate,which was consistent with the expansion of ovary in JD-h-15 after self-pollination. Micromorphological analysis showed that the pollen grains of JD-h-15 were larger than those of the control,while the starch grains in bulbils and bulbs were much smaller than those of the control. In JD-h-15 bulbs,the contents of soluble sugar,total flavonoids and total saponins increased by 42.37%,67.54% and 45.65% respectively,while ascorbic acid,total phenolic acids and total alkaloids decreased by 22.86%,33.02% and 50.41% compared with that in the control. The 3 years’ old JD-h-15 and the control plants were genetically stable after ISSR marker analysis. Compared with the control,JD-h-15 plants experienced the genetic variation and the variation rate of JD-h-15 was 21.30%. These data provided a reference for the improvement of L. lancifolium genetic characters and the cultivation of new germplasm used by colchicine in lilies.

Key words: Lilium lancifolium, genetic improvement, morphological characteristics, active component, ISSR marker