Advances in Plant Flavonoids UDP-glycosyltransferase

doi:10.13560/j.cnki.biotech.bull.1985.2022-0236

Abstract

Abstract:

Flavonoids are important natural products in plants and usually exist in the form of glycosides. Uridine diphosphate glycosyltransferases（UGTs）can modify flavonoids to form a variety of flavonoid glycosides, which are the active substances of flavonoid medicines in many medical plants. In recent years, as more and more plant genomes have been elucidated, a large number of glycosyltransferases involved in flavonoid synthesis have been identified. In this paper, we firstly introduced the structural features and family classification of plant UGTs, and then reviewed the advances in the study of flavonoid UGTs, had a complete summary on the selectivity of modification sites of plant flavonoid UGT in varied families, as well as the specificity of donors and receptors. It is aimed to lay a foundation for investigating the correlation between structure and function of plant flavonoid UGTs as well as the mining and identification of novel flavonoid UGTs.

Key words: flavonoids, biosynthesis, glycosyltransferase, uridine diphosphate glycosyltransferase, gene family

YAO Yu, GU Jia-jun, SUN Chao, SHEN Guo-an, GUO Bao-lin. Advances in Plant Flavonoids UDP-glycosyltransferase[J]. Biotechnology Bulletin, 2022, 38(12): 47-57.

Figures/Tables 5

References 68

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组 Group	UGT家族 UGT family	催化底物 Substrate
A	79，91，94	类黄酮、皂苷和细胞分裂素
B	89，96	油菜素甾醇、萜类、类黄酮和苯甲酸盐等
C	90，97
D	73，98，99，702，703，704，705，729
E	70，71，72，88，706，707	ABA、木纤维素、萜类化合物、苯甲酸盐和类黄酮化合物
F	78，77，714	类黄酮
G	85	萜类和玉米素
H	76，710	生长素、邻氨基苯甲酸盐、花青素、苯丙素类和油菜素甾醇
I	83，712
J	87
N	82
K	86
L	74，75，84	类黄酮、苯丙烷和生长素吲哚乙酸
M	92	细胞分裂素
O	93，722	植物激素和细胞分裂素
P	709，720	萜类
Q	95，716	类黄酮
R	708

组 Group	UGT家族 UGT family	催化底物 Substrate
A	79，91，94	类黄酮、皂苷和细胞分裂素
B	89，96	油菜素甾醇、萜类、类黄酮和苯甲酸盐等
C	90，97
D	73，98，99，702，703，704，705，729
E	70，71，72，88，706，707	ABA、木纤维素、萜类化合物、苯甲酸盐和类黄酮化合物
F	78，77，714	类黄酮
G	85	萜类和玉米素
H	76，710	生长素、邻氨基苯甲酸盐、花青素、苯丙素类和油菜素甾醇
I	83，712
J	87
N	82
K	86
L	74，75，84	类黄酮、苯丙烷和生长素吲哚乙酸
M	92	细胞分裂素
O	93，722	植物激素和细胞分裂素
P	709，720	萜类
Q	95，716	类黄酮
R	708

组Group	UGT家族UGT family	主要修饰位点Modification site
A	79，95，716	GGTs
B	89	7-OH
C	90	4'-OH
D	73	7-OH
E	88，70，71	7-OH（唇形目Lamiales）
F	78	3-OH
L	75，84	5-OH

组Group	UGT家族UGT family	主要修饰位点Modification site
A	79，95，716	GGTs
B	89	7-OH
C	90	4'-OH
D	73	7-OH
E	88，70，71	7-OH（唇形目Lamiales）
F	78	3-OH
L	75，84	5-OH

组 Group	UGT家族 UGT family	基因ID Gene ID	物种 Species	拉丁名 Latin name	基因登录号 Accession No.	修饰位点 Modification site	底物 Substrate
A	79	PhA3RhaT^[40]	矮牵牛	Petunia hybrida	Q43716.1	3-OH	花青素
A	79	AtUGT79B2^[24]	拟南芥	Arabidopsis thaliana	Q9T080.1	3-OH	花青素
A	95	GbUGT716A1^[42]	银杏	Ginkgo biloba	ASK39400.1	3-OH和7-OH为主，也能修饰3'-OH和4'-OH	黄酮、黄酮醇、异黄酮、没食子和没食子酸黄烷醇
A	95	PgUGT95B2^[29]	石榴	Punica granatum	AZB52139.1	4'-OH	黄酮醇和黄酮
A	95	HpUGT95A1^[41]	绿毛山柳菊	Hieracium pilosella	ACB56927.1	3'-OH	黄酮
C	90	HpUGT90A7^[41]	绿毛山柳菊	Hieracium pilosella	EU561019.1	4'-OH为主，也能修饰7-OH	黄酮醇
E	71	FaGT6^[47]	草莓	Fragaria x ananassa	ABB92748.1	3-OH为主，也能修饰7-OH、4'-OH和3'-OH	黄酮醇为主，也能催化羟基香豆素和萘酚
E	70	CsUGT707B1^[22]	红花	Crocus sativus	CCG85331.1	2''-OH	黄酮醇
E	73	FaGT7^[47]	草莓	Fragaria x ananassa	ABB92749.1	3-OH和4'-OH为主，也能修饰7-OH和3'-OH	黄酮醇、羟基香豆素和萘酚
E	88	GmUGT88E19^[46]	大豆	Glycine max	XP_003533968	3'-OH和7-OH	异黄酮
L	84	NmF4' G7GT^[48]	粉蝶花	Nemophila menziesii	BBA68563.1	4'-OH和7-OH	黄酮