Applications and Prospect of Genome Editing Techniques in Precise Potato Molecular Breeding

doi:10.13560/j.cnki.biotech.bull.1985.2019-1272

Abstract

Abstract: After nearly a decade of rapid development,genome editing techniques have become powerful tools for gene function research and precise molecular breeding. These technologies mainly result in the DNA insertion or deletion mutation at the target site by causing double-stranded DNA fracture at these sites and triggering the repair mechanisms of the cells. With the implementation of the national strategy of using potato as staple diet,people’s demand for potato varieties will become more and more multi-faceted,and genome editing will provide efficient and fast technical approaches for directed genetic improvement and precise molecular breeding in potato. In this paper,the principles of three mainstream genome editing techniques were briefly reviewed,their applications in improvement and precise molecular breeding of potato varieties were retrospected,significance and present problems of these techniques in potato precision molecular breeding were discussed,and the future application of genome editing in potato molecular breeding was prospected,aimed at providing reference and new ideas for future potato precision molecular breeding.

Key words: potato, genome editing, precise molecular breeding, application

YE Ming-wang, LI Can-hui, GONG Ming. Applications and Prospect of Genome Editing Techniques in Precise Potato Molecular Breeding[J]. Biotechnology Bulletin, 2020, 36(3): 9-17.

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