Research Progress in S-adenosyl-L-methionine Dependent 3-amino-3-carboxypropyl Utilizing Enzymes

doi:10.13560/j.cnki.biotech.bull.1985.2023-0220

Abstract

Abstract:

S-adenosyl-L-methionine（SAM）is a very important cofactor in biology. It possesses three unstable C-S bonds, which can be selectively cleaved by various SAM-dependent enzymes, catalyzing a wide range of biochemical reactions. Methyltransferases and radical SAM enzymes, which cleave C-S bonds and use methyl and 5'-dexoyadenosine of SAM respectively, have been intensively studied. Over 1.8 million protein sequences of methyltransferases have been deposited in the UniProt database. Radical SAM enzymes have grown into one of the largest enzyme superfamilies, consisting of over 700 000 members. However, enzymes utilizing the 3-amino-3-carboxypropyl（ACP）group of SAM are relatively rarely reported. This paper aims to classify and introduce these enzymes while also provide a prospective research direction for the future.

Key words: S-adenosyl-L-methionine, 3-amino-3-carboxypropyl, reaction mechanism, methyltransferase, non-canonical radical SAM enzyme

HE Jia-le, DONG Min. Research Progress in S-adenosyl-L-methionine Dependent 3-amino-3-carboxypropyl Utilizing Enzymes[J]. Biotechnology Bulletin, 2023, 39(12): 56-70.

Figures/Tables 8

References 86

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名称 Name	底物 Substrate	反应类型 Reaction
TYW2^[5]	4-去甲基丫苷	ACP转移反应（极性机理）
Tsr3^[6]	N¹-甲基假尿苷	ACP转移反应（极性机理）
YfiP^[7]	尿苷
NAS^[8]	氮杂环丁烷2-甲酸
MtNAS^[9]	谷氨酸
CntL^[10]	组氨酸
SPDS、SPMS^[11]	多胺
MccD^[12]	肽酰腺苷酸
NAT^[13]
BtaA^[14]	二酰甘油
Dph2^[15]	蛋白质翻译延伸因子2	ACP转移反应（自由基机理）
SbzP^[16]	β-NAD	ACP参与的环化反应
ACS、GnmY、ClbH^[17]	氨基环丙基-1-甲酸	ACP自身的环化反应
VioH、AzeJ^[18]	氮杂环丁烷2-甲酸

名称 Name	底物 Substrate	反应类型 Reaction
TYW2^[5]	4-去甲基丫苷	ACP转移反应（极性机理）
Tsr3^[6]	N¹-甲基假尿苷	ACP转移反应（极性机理）
YfiP^[7]	尿苷
NAS^[8]	氮杂环丁烷2-甲酸
MtNAS^[9]	谷氨酸
CntL^[10]	组氨酸
SPDS、SPMS^[11]	多胺
MccD^[12]	肽酰腺苷酸
NAT^[13]
BtaA^[14]	二酰甘油
Dph2^[15]	蛋白质翻译延伸因子2	ACP转移反应（自由基机理）
SbzP^[16]	β-NAD	ACP参与的环化反应
ACS、GnmY、ClbH^[17]	氨基环丙基-1-甲酸	ACP自身的环化反应
VioH、AzeJ^[18]	氮杂环丁烷2-甲酸