Effects of Bacillus pumilus on the Intestinal Inflammation and Barrier Function of Goat Kids

doi:10.13560/j.cnki.biotech.bull.1985.2023-0498

Abstract

Abstract:

【Objective】This experiment was conducted to investigate the effects of Bacillus pumilus on growth performance, diarrhea, serum indexes, colonic tissue morphology, inflammatory cytokines and mucosal protein contents of goat kids. 【Method】Thirty-six one-day-old male Saneng dairy goat kids with birth weight of about 3kg were randomly allocated to 4 groups with 9 replicates per group and 1 goat kid per replicate. The goat kids were fed milk replacer containing Bacillus pumilus solution 0（CON group）, 1 mL（BP1 group）, 5 mL（BP5 group）and 10 mL（BP10 group）every day, respectively. The viable count of B. pumilus was 1×10⁸ CFU/mL. The experiment lasted for 14 d. 【Result】Feeding B. pumilus had no significant effect on the final weight, average daily gain, average daily feed intake and feed to gain ratio of goat kids（P > 0.05）, but decreased the fecal score and diarrhea frequency（P < 0.05）. The content of aspartate aminotransferase in the serum of BP1 group was lower than that of control group（P < 0.05）, and the content of diamine oxidase was lower than that of control group and BP5 group（P < 0.05）. Compared with control group, the content of D-lactic acid decreased in the other three groups（P < 0.05）. The thickness of colonic muscle layer increased in BP1 group（P < 0.05）. Compared with the control group, the contents of IL-1β, IL-6 and TNF-α in BP1 group, BP5 group and BP10 group decreased（P < 0.05）. The contents of IL-10, TGF-β, IFN-γ, PPAR-γ, MUC2, Claudin-1, Claudin-4, Occludin and ZO-1 increased（P < 0.05）, and neutrophil cell infiltration in colon tissue decreased. 【Conclusion】Feeding Bacillus pumilus to goat kids may reduce the frequency of diarrhea, relieve inflammatory reaction, and enhance mucosal barrier function of goat kids, but there is no significant effect on growth performance.

Key words: Bacillus pumilus, goat kids, growth performance, inflammation of the colon, tight junction protein

JIAO Shuai, FU Yu-ze, CUI Kai, ZHANG Ji-xian, WANG Jie, BI Yan-liang, DIAO Qi-yu, ZHANG Jian-xin, ZHANG Nai-feng. Effects of Bacillus pumilus on the Intestinal Inflammation and Barrier Function of Goat Kids[J]. Biotechnology Bulletin, 2024, 40(1): 344-352.

Figures/Tables 9

References 31

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营养水平Nutrient level	含量Content
干物质 DM	95.00%
粗蛋白质 CP	22.00%
粗脂肪 EE	24.00%
乳糖 Lactose	35.00%
粗灰分 Ash	9.00%
粗纤维 CF	0.20%
钙 Ga	0.72%
磷 P	0.44%
赖氨酸 Lys	1.70%
氯化钠 NaCl	1.00%
维生素A Vitamin A	25 000 IU/kg

营养水平Nutrient level	含量Content
干物质 DM	95.00%
粗蛋白质 CP	22.00%
粗脂肪 EE	24.00%
乳糖 Lactose	35.00%
粗灰分 Ash	9.00%
粗纤维 CF	0.20%
钙 Ga	0.72%
磷 P	0.44%
赖氨酸 Lys	1.70%
氯化钠 NaCl	1.00%
维生素A Vitamin A	25 000 IU/kg

项目 Item	组别Group				SEM	P值 P-value
项目 Item	CON	BP1	BP5	BP10	SEM	处理Treatment	线性Linear	二次Quadratic
初重 Initial BW/kg	2.83	2.83	3.00	2.88	0.305	0.932	0.726	0.776
末重 Final BW/kg	4.38	4.36	4.66	4.52	0.482	0.918	0.646	0.853
平均日增重ADG/g	111.07	109.56	118.57	116.79	19.192	0.958	0.670	0.992
平均日采食ADFI/g	130.25	124.14	135.74	122.45	12.039	0.679	0.759	0.676
料重比 F/G	1.19	1.17	1.29	1.19	0.173	0.897	0.853	0.753
粪便评分 Fecal score	1.99^a	1.40^b	1.40^b	1.49^b	0.165	0.003	0.008	0.007
腹泻频率 Diarrhea frequency/%	56.25^a	26.79^b	28.72^b	30.50^b	9.378	0.012	0.017	0.026

项目 Item	组别Group				SEM	P值 P-value
项目 Item	CON	BP1	BP5	BP10	SEM	处理Treatment	线性Linear	二次Quadratic
初重 Initial BW/kg	2.83	2.83	3.00	2.88	0.305	0.932	0.726	0.776
末重 Final BW/kg	4.38	4.36	4.66	4.52	0.482	0.918	0.646	0.853
平均日增重ADG/g	111.07	109.56	118.57	116.79	19.192	0.958	0.670	0.992
平均日采食ADFI/g	130.25	124.14	135.74	122.45	12.039	0.679	0.759	0.676
料重比 F/G	1.19	1.17	1.29	1.19	0.173	0.897	0.853	0.753
粪便评分 Fecal score	1.99^a	1.40^b	1.40^b	1.49^b	0.165	0.003	0.008	0.007
腹泻频率 Diarrhea frequency/%	56.25^a	26.79^b	28.72^b	30.50^b	9.378	0.012	0.017	0.026

项目 Item	组别Group				SEM	P值 P-value
项目 Item	CON	BP1	BP5	BP10	SEM	处理Treatment	线性Linear	二次Quadratic
谷丙转氨酶ALT/（U·L^-1）	43.51	34.26	39.07	37.78	3.531	0.104	0.281	0.127
谷草转氨酶AST/（（U·L^-1）	77.53^a	63.56^b	69.68^ab	70.85^ab	4.552	0.047	0.345	0.029
二胺氧化酶DAO/（U·L^-1）	11.66^a	9.43^b	11.23^a	10.63^ab	0.684	0.022	0.556	0.109
D-乳酸 LD/（mmol·L^-1）	8.04^a	6.38^b	6.72^b	6.91^b	0.282	<0.001	0.003	<0.001