Abstract:

【Objective】Evaluate preexisting H5Nx cross-reactivity immune memory to provide theoretical data for the prevention and treatment of human H5 avian influenza viruses and the development of broad-spectrum vaccines.【Method】Bioinformatics methods were used to screen the common conserved cross-reactivity CD4+ T cell epitopes of human H5Nx avian influenza viruses and seasonal influenza viruses, and further analysis of the nested CD8+ T cell epitopes and B cell epitopes of conserved epitopes and their coverage among the Chinese population.【Result】92.3%（513/555）of H5Nx CD4+ T cell epitopes were cross-reactivity in seasonal influenza A viruses, of which 172 epitopes nested CD8+ T cell epitopes and 5 epitopes nested B cell epitopes . These epitopes and the corresponding HLA-DRB1 alleles have a strong coverage rate of 88.65% in the world population.【Conclusion】Due to repeated exposure to seasonal influenza A viruses, there is a certain level of H5Nx cross-reactivity immune memory in the population that can provide partial protection from H5Nx infection.

Key words: H5Nx influenza virus, co-conserved, cross-reactivity, CD4+ T-cell epitopes, nested epitopes.