Biotechnology Bulletin ›› 2012, Vol. 0 ›› Issue (12): 139-143.

• Study Report • Previous Articles     Next Articles

Expression of Plant des-pGlu1-Brazzein Gene in Alcohol OxidasedefectiveStrain of Pichia pastoris and Analysis of Its Biological Activity

Xu Tingting, Chen Ruibo, Bao Yuanyuan, Zhao Weidong, Zheng Zhenyu, Li Chunli   

  1. Department of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002
  • Received:2012-05-11 Revised:2013-01-25 Online:2012-12-26 Published:2013-02-06

Abstract: Digested by Bgl Ⅱ, the linear recombinant expression vector pPIC9K-Bra was transformed into Pichia pastoris GS115 byelectric shock to produce alcohol oxidase -defective expression strain GS115-pPIC9K-Bra. The strain was identified by PCR and induced by 0.5%methanol. The results indicated that the secreted target protein accounted for 95% of total protein in supernatant fluid and showed biologicalactivity. The alcohol oxidase -defective expression strain GS115-pPIC9K-Bra was successfully constructed which was foundation for furtherstudy.