Biotechnology Bulletin ›› 2012, Vol. 0 ›› Issue (12): 82-87.

• Study Report • Previous Articles     Next Articles

Screening on Recipient Material for Agrobacterium-mediatedTransformation in Maize

Jiang Huiquan1,2, Ren Wen1, Xu You2, Zhang Zhifang1, Chen Hao1, He Haohua2, Zhao Jiuran1, Liu Ya1   

  1. 1Maize Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097 ;2College of Science,Jiangxi AgricultureUniversity,Nanchang 330000
  • Received:2012-05-05 Revised:2013-01-25 Online:2012-12-26 Published:2013-02-06

Abstract: At present, Agrobacterium mediated genetic transformation in maize is widely used in the world. A good recipient material isvery important for the transformation system. In this study, 14 new inbreed lines were used to choose the suitable recipient material by analysingembryogenic Type Ⅱ callus induction, plant regeneration rate and GUS transient expression. Zpm2 and zpm5 were finally screened for maizetransformation system. In the study of mature embryos callus induction, mature embryos callus induction rate can predict the growth status ofimmature embryos callus and their callus induction show a positive correlation.