Profile of Drought Resistance of Tomato Transcription FactorSlDREB2 Gene Mediated by VIGS

Abstract

Abstract: SlDREB2 gene was isolated by yeast one-hybrid system using DRE element of rd29A promoter through hybridization withtomato seedlings（Lichun）cDNA library, and it is a transcription factor belonging to EREBP family. In this study, the VIGS virus vectors wereconstructed, and transformed into the GV3101 Agrobacterium strains, and the resulting recombinants carrying pBINTRB6, pTV00-SlDREB2-1and pTV00-SlDREB2-2 vectors were used to infect leaves of tomato plants respectively by injection of liquid filling respectively. All the plantsinfected after 7 days were identified by viral DNA testing and the physiological index of wild-type strains of tomato plants as well as infectedplants successfully were determined under drought stress for three weeks, and Real-time qPCR was performed to analyze expression profileof the SlDREB2 transcription factor. Data showed that the contents of proline and soluble sugar in wild tomato plants were higher than that oftomato plants infected by VIGS virus, and malondialdehyde levels in wild plants were lower than that of plants infected by VIGS virus. Whenall tomato plants stressed by drought for three weeks were re-watered for one week, the contents of proline and soluble sugar in wild tomatoplants were lower than that of tomato plants by VIGS virus infection, and the contents of malondialdehyde in wild plants were higher than that ofplants infected by VIGS virus infection. Real-time qPCR results indicates that the expression of SlDREB2 in plants by VIGS virus infection waslower significantly than that of in wild type plants under drought stress, indicating that 500 bp of specific fragment at the end of SlDREB2 geneconservative domain has better silence effect, and the expression of SlDREB2 mediated by VIGS was inhibited by drought stress, and indirectlyimplying that the SlDREB2 gene can be a valuable candidate gene for improving crop varieties with drought resistance.

Yu Guohong, Ma Xuefeng, Xu Na, Xin Shichao, Cheng Xianguo . Profile of Drought Resistance of Tomato Transcription FactorSlDREB2 Gene Mediated by VIGS[J]. Biotechnology Bulletin, 2012, 0(12): 93-100.

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