Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (1): 68-72.

• Techniques and methods • Previous Articles     Next Articles

Research Progress on the Detection Methods of D-amino Acids

Li Huixin, Ju Jiansong, Zhang Linlin, Xu Shujing   

  1. College of Life Science,Hebei Normal University,Shijiazhuang 050024
  • Received:2012-08-21 Revised:2013-01-31 Online:2013-01-30 Published:2013-01-30

Abstract: As we all know there are 20 different kinds of basic amino acids in nature. In addition to glycine, each amino acid has twoenantiomers D-and L-type. D-amino acids have long been considered to be unique to microorganisms, which utilize some D-amino acids toconstruct the peptidoglycan layer of their cell wall. However, several D-amino acids have been detected in many organisms including mammalsbecause of the advance of analytical techniques in the last two decades. It was realized that D-amino acids might play significant roles in livingthings and as the important component of medicine, pesticide and food. In order to get a better analyzing and understanding the important role ofD-amino acids, the analytical methods for D-amino acids detection are constantly developing. Herein, several methods include enzymatic method,Gas chromatography, HPLC and biosensor assay were introduced.

Key words: D-amino acid , Detection , HPLC , Biosensor