Cell Model Construction of Regulated Hepatitis B Virus X Gene Expression

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (2): 140-146.

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Cell Model Construction of Regulated Hepatitis B Virus X Gene Expression

Bai Helu Liu Rui Zhu Naishuo

（Department of Microbiology and Microbial Biotechnology，School of Life Science，Fudan University，Shanghai 200433）

Received:2012-07-17 Revised:2013-02-27 Online:2013-02-26 Published:2013-02-27
Contact: 朱乃硕，男，教授，博士生导师，研究方向：感染与分子免疫学；E-mail ：nzhu@fudan.edu.cn

Abstract

Abstract: An HBx inducible expression plasmid carrier was constructed by Lentil-XTM Tet-On Advanced Inducible Expression System, and rt-TA gene virus particles and HBx gene virus particles were synthesized by lentiviral packing cell line HEK293T cells, with the titer reaching 108 LPs/mL. When HepG2 cell lines were infected by the two virus particles and selected by G418 and puromycin antibiotics, stable cell line with HBx gene could be constructed. After adding Dox into the stable cell line, the inducible expression of HBx gene could be proved by RT-PCR and Western blot. Our study provides an ideal experiment model for further research on the roles of HBx in HBV infection and the host immune tolerance.

Key words: Hepatitis B virus, Lentiviral expression system, Tetracycline regulation, Inducible expression

Bai Helu, Liu Rui, Zhu Naishuo . Cell Model Construction of Regulated Hepatitis B Virus X Gene Expression[J]. Biotechnology Bulletin, 2013, 0(2): 140-146.

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Cell Model Construction of Regulated Hepatitis B Virus X Gene Expression

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