Establishment of the Screening Oligonucleotide Microarray of Apscaviroid in Genus Level

Abstract

Abstract: Apscaviroid is a significant plant viroid genus and has not screening method available at present. For the establishment of the screening oligonucleotide microarray of Apscaviroid in genus level, thirty-five genus specific probes from the sequences of Apscaviroid viroid were designed. Microarray was constructed based on these probes and identificated with Apple scar skin viroid and Citrus dwarfing viroid standard samples. Results showed a high specificity for detection of Apscaviroid viroid and the same detection sensitivity with RT-PCR. The microarray can be used to screen and provide technical support of quarantine and control for Apscaviroid viroid.

Key words: Apscaviroid, Genus level probe, Oligonucleotide microarray

Zhang Yongjiang, Xin Yanyan, Zhu Shuifang, Li Shifang. Establishment of the Screening Oligonucleotide Microarray of Apscaviroid in Genus Level[J]. Biotechnology Bulletin, 2013, 0(2): 151-156.

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