Advantages of the Dynamic Model in Real-time PCR Data Processing

Abstract

Abstract: In recent years, Real-time PCR with its advantages in the quantitation of gene is widely used, the development of equipment ensure the accuracy of raw data, however the reliability of quantitative result highly depends on the PCR efficiency and the mathematical model on which the quantitative methods are based, The quantitative result processed with different mathematical models will be quite different. In this paper, the sample was 5-fold diluted for gaining the raw data of quantitative PCR, and the same raw data was processed by three kinds of mathematical models, including 2 －△△ Ct model, relative standard curve model, dynamic model. And the results demonstrate the dynamic model has a good prospect.

Key words: Quantitative, PCR 2 －△△ Ct model, Relative standard curve model, Dynamic model

Liu Yanli, Li Xu, Su Zhencheng, Xu Mingkai, Zhang Huiwen. Advantages of the Dynamic Model in Real-time PCR Data Processing[J]. Biotechnology Bulletin, 2013, 0(2): 157-162.

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