Optimization and Construction of the Intracellular and Extracellular Proteomic Map of Lysobacter yanansis sp. nov.

Abstract

Abstract: In this study, we separated the extracellular protein excreted by Lysobacter yanansis sp. nov. through the two-dimensional （2-D）electrophoresis, and analyzed the effect of isoelectric focusing programs and loading volumes on 2-D proteomic maps. The results showed that a clearly 2-DE map was obtained when the 150 μg loading volumes and isoelectric focusing programs Ⅱ were used. Furthermore, 2-D proteomic maps of extract intracellular and extracellular protein from Lysobacter yanansis sp. nov. were constructed. The separated protein spots in gels were analyzed by PDQuest. 100 spots of extracellular proteins and about 204 spots of intracellular proteins had been separated by 2-DE.

Key words: Lysobacter, Two-dimensional electrophoresis（2-DE）, Biodefence

Wang Feifei, Wu Kunyi, Guo Ling, Cui Langjun, Ren Jing . Optimization and Construction of the Intracellular and Extracellular Proteomic Map of Lysobacter yanansis sp. nov.[J]. Biotechnology Bulletin, 2013, 0(4): 140-146.

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