Screening and Identifying of GPS2 ：a Novel Interactive Protein of LOH12CR1

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (4): 201-205.

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Screening and Identifying of GPS2 ：a Novel Interactive Protein of LOH12CR1

Wang Yongjun¹, Wang Yingwei², Shi Xiaoliu¹, Lin Ruoheng¹, Zhang Wenting¹, Shen Hongwei¹

1. The Second Xiangya Hospital of Central South University，Changsha 410011 ；2. The People’s Hospital of Liuzhou City，Liuzhou 050000

Received:2012-10-19 Revised:2013-04-22 Online:2013-04-22 Published:2013-04-22

Abstract

Abstract: Loss of heterozygosity of the short arm of chromosome 12 is a frequently observed in a wide variety of haematological malignancies and solid tumours, suggesting the presence of tumour suppressor genes in this region. LOH12CR1 is one of the genes identified in 12p12. To explore the normal function of LOH1CR12, yeast two-hybrid system was used to screen proteins interacting with LOH1CR12, and the transcriptional regulator GPS2 was identified as a LOH1CR12- interacting protein. Furthermore, GPS2 was confirmed to interact with LOH1CR12 in vivo in HEK-293 cell by co-immunoprecipitation, and the co-localization of GPS2 and LOH1CR12 in nucleus was observed by immunofluorescent co-localization.

Key words: LOH12CR1, GPS2, Yeast two-hybrid system, Interactive protein, Co-immunoprecipitation

Wang Yongjun, Wang Yingwei, Shi Xiaoliu, Lin Ruoheng, Zhang Wenting, Shen Hongwei, . Screening and Identifying of GPS2 ：a Novel Interactive Protein of LOH12CR1[J]. Biotechnology Bulletin, 2013, 0(4): 201-205.

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Screening and Identifying of GPS2 ：a Novel Interactive Protein of LOH12CR1

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