Preparation and Analysis on Biological Characteristics of Monoclonal Antibodies Against CP4-EPSPS for Sandwich ELISA

Abstract

Abstract:

In order to obtain specific monoclonal antibody against CP4-EPSPS protein for sandwich ELISA, the CP4-EPSPS gene was cloned into a bacterial expression vector and transformed into E.coli strain Rossetta. The target protein was expressed efficiently. After purification, the target protein was obtained. Purified CP4-EPSPS was used to immunize BALB/c mice and two monoclonal antibodies（1A5 and 8A3）were generated. These antibodies can recognize nature and denatured CP4-EPSPS. Overlay ELISA showed that they recognize different epitopes on CP4-EPSPS, so they can be used in sandwich ELISA to detect CP4-EPSPS.

Key words: CP4-EPSPS, Protein expression, Monoclonal antibody, Sandwich ELISA

Li Zhongpeng, Yu Hansong, Hu Yaohui, Shi Shengfeng, Liu Yunhui, Duan Yongjie, Li Xiaoyu, Wang Yongzhi, Li Qiyun. Preparation and Analysis on Biological Characteristics of Monoclonal Antibodies Against CP4-EPSPS for Sandwich ELISA[J]. Biotechnology Bulletin, 2013, 0(4): 206-209.

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