Genetic Characteristics of GOLA-DQA1 Gene at Exon2 in Hexi Cashmere Goat

Abstract

Abstract: Genetic Characteristics of GOLA-DQA1 gene at exon2 and the association of alleles with pregnancy in Hexi cashmere goat were analyzed using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing technology. Nine alleles were identified at GOLA-DQA1 gene at exon2 in Hexi cashmere goat and 3 new DQA1 alleles were identified (GOLA-DQA1*B, GOLA-DQA1*E and GOLA-DQA1*H). Sequence analysis indicated that there were 42 single nucleotide polymorphism sites, which occupied 12.65% of exon 2 sequences which include twenty three transition sites, thirteen transversion sites, five coexist sites and one insertion site. Correlation analysis showed significant statistic difference between different alleles (P<0.05). Frequencies of GOLA-DQA1*A were significantly in abortion group higher than those in normal group (P<0.05). In contrast, GOLA-DQA1*H allelic frequencies in abortion group were significance lower than those in normal group (P<0.05). The result suggested that the allele GOLA-DQA1*A might associated with abortion and GOLA-DQA1*H might related to pregnancy protection in Hexi Cashmere Goat.

Key words: GOLA-DQA1 gene, Hexi cashmere goat, Genetic characteristic, PCR-SSCP, Abortion

Chai Wenqiong, Wang Jiqing, Hu Jiang, Liu Xiu, Li Shaobin, Luo Yuzhu. Genetic Characteristics of GOLA-DQA1 Gene at Exon2 in Hexi Cashmere Goat[J]. Biotechnology Bulletin, 2013, 0(5): 121-125.

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