Single Nucleotide Polymorphism Analysis of Duck Adiponectin Gene

Abstract

Abstract: It was to study the Adiponectin gene polymorphism in duck population. The single nucleotide polymorphism(SNP) of Adiponectin gene T523C SNP on exon2 of Adiponectin was investigated in seven national duck breeds, including Beijing duck, Gaoyou duck, Putian black duck, Liancheng white duck, Shaoxing duck, Youxian Sheldrake and Jianchang duck by PCR-LDR method. And the gene frequency and genotype frequency distribution was analyzed by Hardy-Weinberg equilibrium. Results showed TT, CC and TC genotypes of Adiponectin gene T523C were all detected in seven populations. TC was dominant in six populations except Liancheng white duck, TT was dominant only in Liancheng white duck. T gene frequency was higher than C gene frequency in Beijing duck, Putian black duck, Liancheng white Duck and Youxian Sheldrake. At the other hand, C gene frequency was higherter than T gene frequency in Gaoyou duck, Shaoxing duck and Jianchang duck. The gene at T523C of Adiponectin gene is in genetic balance in 7 duck populations (P>0.05), which will provide an experimental basis on duck molecular genetics.

Key words: Duck, Adiponectin gene, Ligase detection reaction, Single nucleotide polymorphism

Zhang Yinhong, Li Huifang, Zhu Wenqi. Single Nucleotide Polymorphism Analysis of Duck Adiponectin Gene[J]. Biotechnology Bulletin, 2013, 0(5): 126-129.

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