Analysis for Differentially Expressed Proteins of HSC-T6 Cell Affected by Nerve Growth Factor

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (5): 130-136.

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Analysis for Differentially Expressed Proteins of HSC-T6 Cell Affected by Nerve Growth Factor

Xu Jin¹, Zhang Xuerong¹, Hu Rentong¹, Luo Xiaoling², Chen Ying¹, Lin Xing¹, Liao Ming¹, Fu Rao¹, Fu Daoying¹

(1. Medical Sciences Experimental Center of Guangxi Medical University，Nanning 530021；2. Cancer Hospital of Guangxi Medical University，Nanning 530021)

Received:2012-11-20 Revised:2013-05-24 Online:2013-05-24 Published:2013-05-24
About author:张学荣，男，教授，硕士生导师，研究方向：蛇毒药物应用，肝纤维化；E-mail：zxrsv@sina.com

Abstract

Abstract: It was to develop the 2-DE profiles of proteome from hepatic stellate cell and preliminarily analyze the affect of NGF on the protein expression of HSC-T6 cell. We established groups of control and NGF (4、8、16 mg/L), respectively, then acting on the HSC-T6. The differential expression of proteins were analyzed by imaging analysis and MALDI-TOF-MS after the total protein was extracted from the blank control group and NGF treated by 2-DE. The differential protein spots were classified with GO and analyzed with Signal transduction pathway. Results showed that HSC-T6 cell proliferation is inhibited evidently. Forty-seven differentially expressed protein were found in the proteome profile analysis of these two types of blank control group and NGF treated, among which 22 protein spots were up-regulated and 25 protein spots were down-regulated in the HSC-T6 treated by NGF, part of the different expression of protein is increasing with increasing of NGF concentration and part is decreasing with increasing of NGF concentration. Eighteen protein spots with the differential expressions more than 1.8 times were analyzed by peptide mass fingerprint (PMF) and thirteen differentially expressed proteins were found to be related with cell signal transduction, cell proliferation and oxidative stress. The cobra venom NGF can change the expression of the different protein expression of HSC-T6 cell in signaling pathway, providing a theoretical basis for the antifibrotic mechanism of NGF at protein level.

Key words: NGF, HSC-T6, Proteomics 2-DE, MALDI-TOF-MS

Xu Jin, Zhang Xuerong, Hu Rentong, Luo Xiaoling, Chen Ying, Lin Xing, Liao Ming, Fu Rao, Fu Daoying. Analysis for Differentially Expressed Proteins of HSC-T6 Cell Affected by Nerve Growth Factor[J]. Biotechnology Bulletin, 2013, 0(5): 130-136.

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Analysis for Differentially Expressed Proteins of HSC-T6 Cell Affected by Nerve Growth Factor

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