Study on Sites of the Tolerate Peptide Insertion in the Fluorescent Protein of mCherry

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (5): 144-148.

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Study on Sites of the Tolerate Peptide Insertion in the Fluorescent Protein of mCherry

Liang Junting^{1, 2}, Li Luzhi², Chen Shaopeng², Jiao Zhen¹

(1. Henan Provincial Key Laboratory of Ion Beam Bioengineering，Zhengzhou University，Zhengzhou 450052；2. Institute of Technical Biology & Agriculture Engineering，Chinese Academy of Sciences，Hefei 230031)

Received:2012-11-12 Revised:2013-05-24 Online:2013-05-24 Published:2013-05-24
About author:焦浈，女，博士，副教授，研究方向：作物遗传育种；E-mail：jiaozhen@zzu.edu.cn

Abstract

Abstract: The fluorescent proteins(FPs)and their color variants have been widely used in cell biology, molecular biology and medicine. Various new technologies based on these proteins such as bimolecular fluorescence complementation (BiFC), fluorescence resonance energy transfer (FRET)-based techniques and optical highlighter are enlightening the whole biology technology. In this paper, we used transposon-based mutagenesis and flow cytometry to verify thirteen tolerate sites of mCherry with five amino acids inserted which fluoresced red. The inserted variants with fluorescence can be served for BiFC and provide more selective sites for designing of bio-sensors.

Key words: Red fluorescent protein, Short-peptide, Inserted sites

Liang Junting, Li Luzhi, Chen Shaopeng, Jiao Zhen. Study on Sites of the Tolerate Peptide Insertion in the Fluorescent Protein of mCherry[J]. Biotechnology Bulletin, 2013, 0(5): 144-148.

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Study on Sites of the Tolerate Peptide Insertion in the Fluorescent Protein of mCherry

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