Breeding of High Epothilone B-producing Strains by Genome Shuffling

Abstract

Abstract: The mutation of Sorangium cellulosum AHB125 was induced using the classical UV-mutation method plus selection pressures of 2% sodium acetate. Two mutants, SC4-14 and SC4-56, were used as original strains to screen and breed high Epothilone B-producing strains by genome shuffling and explore the basic rule of genome shuffling in strain breeding. Five hereditarily stable strains with high Epothilone B production were obtained after four cycles of genome shuffling. Strain F4-28 yielded 67.4 mg/L of Epothilone B, which was 8-fold higher than that of the initial strain S. cellulosum AHB125 and 4 to 5-fold higher than the mutants used as the original strains for genome shuffling. In addition, the fermentation period of F4-28 was one day shorter than that of SC4-56. The technique of genome shuffling was an efficient method to screen and breed high Epothilone B-producing strains.

Key words: Epothilone, B, Sorangium cellulosum, AHB125, Genome shuffling

Wang Dahong, Liu Fei. Breeding of High Epothilone B-producing Strains by Genome Shuffling[J]. Biotechnology Bulletin, 2013, 0(5): 155-160.

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