Responses of Rubisco and Rubisco Activase in Different Resistant Tobacco Strains to Brown Spot Stress

doi:10.13560/j.cnki.biotech.bull.1985.2023-0278

Abstract

Abstract:

Using resistant and susceptible tobacco strains JYH and CBH as test materials, the effects of brown spot stress on the photosynthesis and activities of ribulose-1,5-bisphosphate carboxylase/oxygenase（Rubisco）and ribulose-1,5-diphosphate carboxylase/oxygenase activase（RCA）and their gene expressions were analyzed. The results showed that, there were obvious inhibition effects on a series of photosynthetic parameters and the activities, expression levels and protein contents of Rubisco and RCA in JYH at 9 d of brown spot stress. Meanwhile, tobacco brown spot stress notably reduced the photosynthesis and activities of Rubisco and RCA in CBH, in addition, the relative expression of Rubisco large and small subunits genes（rbcL and rbcS）and RCA genes（rca）and their protein contents all decreased under stress condition. In comparison with CBH, the activities, expression levels and protein contents of Rubisco and RCA in JYH were all significantly higher at the stress treatment of tobacco brown spot. The correlation analysis results also indicated that Pn had significant and extremely significant positive correlation with the activities, gene expression of Rubisco and RCA, respectively. These results suggest that the resistant strain JYH can maintain higher values of P_n, Rubisco and RCA activities under tobacco brown spot stress, which account for the resistance to brown spot stress.

Key words: tobacco brown spot, photosynthesis, Rubisco, RCA, gene expression, correlation analysis, resistant difference, strain

YANG Zhi-xiao, HOU Qian, LIU Guo-quan, LU Zhi-gang, CAO Yi, GOU Jian-yu, WANG Yi, LIN Ying-chao. Responses of Rubisco and Rubisco Activase in Different Resistant Tobacco Strains to Brown Spot Stress[J]. Biotechnology Bulletin, 2023, 39(9): 202-212.

Figures/Tables 6

References 48

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基因 Gene	正向引物Forward primer（5'-3'）	反向引物Reverse primer（5'-3'）
rbcL	TTACAAAGGGCGATGCTACC	GAACCCAAATACGTTACCTACAATG
RbcS	GCTTCCTCTGTTCTTTCCTCTG	GCAATGGAAGTGATGTCAAGG
Rca	AGCAGCAGAAATCATCAG	ATTGTCCAGGGAAGGTAT
Actin	CCTGAGGTCCTTTTCCAACCA	GGATTCCGGCAGCTTCCATT

基因 Gene	正向引物Forward primer（5'-3'）	反向引物Reverse primer（5'-3'）
rbcL	TTACAAAGGGCGATGCTACC	GAACCCAAATACGTTACCTACAATG
RbcS	GCTTCCTCTGTTCTTTCCTCTG	GCAATGGAAGTGATGTCAAGG
Rca	AGCAGCAGAAATCATCAG	ATTGTCCAGGGAAGGTAT
Actin	CCTGAGGTCCTTTTCCAACCA	GGATTCCGGCAGCTTCCATT

指标 Index	P_n	rbcL 相对表达量 rbcL relative expression	rbcS相对表达量 rbcS relative expression	rca相对表达量 rca relative expression	rbcL含量 Content of rbcL	rbcS含量 Content of rbcS	rca含量 Content of rca	Rubisco活化状态 Activation state of Rubisco	Rubisco 初始活性 Initial activity of Rubisco	Rubisco总活性 Total activity of Rubisco
rbcL相对表达量 rbcL relative expression	0.996**
rbcS相对表达量 rbcS relative expression	0.989**	0.427
rca相对表达量 rca relative expression	0.991**	0.193	0.704
rbcL含量 Content of rbcL	0.375	0.743	0.588	0.611
rbcS含量 Content of rbcS	0.442	0.347	-0.623	-0.194	0.927*
rca含量 Content of rca	0.296	0.204	0.037	0.974**	0.492	0.613
Rubisco活化状态 Activation state of Rubisco	0.926*	0.938*	0.198	0.427	0.128	0.327	0.979**
Rubisco初始活性 Initial activity of Rubisco	0.917*	0.962*	0.529	0.947*	-0.384	0.535	0.993**	0.584
Rubisco总活性 Total activity of Rubisco	0.943*	0.987**	-0.394	0.919*	0.456	0.136	0.982**	0.371	0.603
RCA活性 Activity of RCA	0.932*	0.449	0.438	0.996**	0.295	0.477	0.438	0.149	0.582	0.497

指标 Index	P_n	rbcL 相对表达量 rbcL relative expression	rbcS相对表达量 rbcS relative expression	rca相对表达量 rca relative expression	rbcL含量 Content of rbcL	rbcS含量 Content of rbcS	rca含量 Content of rca	Rubisco活化状态 Activation state of Rubisco	Rubisco 初始活性 Initial activity of Rubisco	Rubisco总活性 Total activity of Rubisco
rbcL相对表达量 rbcL relative expression	0.996**
rbcS相对表达量 rbcS relative expression	0.989**	0.427
rca相对表达量 rca relative expression	0.991**	0.193	0.704
rbcL含量 Content of rbcL	0.375	0.743	0.588	0.611
rbcS含量 Content of rbcS	0.442	0.347	-0.623	-0.194	0.927*
rca含量 Content of rca	0.296	0.204	0.037	0.974**	0.492	0.613
Rubisco活化状态 Activation state of Rubisco	0.926*	0.938*	0.198	0.427	0.128	0.327	0.979**
Rubisco初始活性 Initial activity of Rubisco	0.917*	0.962*	0.529	0.947*	-0.384	0.535	0.993**	0.584
Rubisco总活性 Total activity of Rubisco	0.943*	0.987**	-0.394	0.919*	0.456	0.136	0.982**	0.371	0.603
RCA活性 Activity of RCA	0.932*	0.449	0.438	0.996**	0.295	0.477	0.438	0.149	0.582	0.497