Identification of Polyphasic Taxonomy for a Chelatococcus sp. HB-4

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (5): 165-170.

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Identification of Polyphasic Taxonomy for a Chelatococcus sp. HB-4

Wu Gang¹, Liu Yang², Li Qing¹, Du Huijing², You Jing¹, Li Hong², Ke Congyu¹, Zhang Xin², Yu Jiliang¹, Zhao Ting²

(1. Petroleum Production Engineering Research Institute of Huabei Oilfield Company，Renqiu 062552；2. China Center of Industrial Culture Collection，China National Research Institute of Food and Fermentation Industries，Beijing 100027)

Received:2012-12-31 Revised:2013-05-24 Online:2013-05-24 Published:2013-05-24
About author:李青，女，工程师，研究方向：微生物采油；E-mail：cyy_liqing@petrochina.com.cn

Abstract

Abstract: Abacterial strain HB-4 was isolated from stratum water in Huabei Oilfield, and identificated by polyphasic taxonomy basing on morphologic and physiologic methods, chemical composition analysis, genetic characteristicstest and 16S rRNA gene phylogenetic analysis. The result showed that the strain was a member of the genus Chelatococcus, and was belong to the known species of Chelatococcus daeguensis. 37℃ was its optimum growth temperature. The major polar lipids presented in strain HB-4 were DPG, PE, PG and PC. Major fatty acids were C_18：1 ω7c (47.01%)and C_19：0 cyclo ω8c(21.83%), and ubiquinone Q-10 was the predominant quinone. HB-4 had enzyme activity of oxidase, catalase, esterase (C4), Esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, chymotrypsin, acid phosphatase and naphthol-AS-BI-phosphate hydrolase.

Key words: Huabei, Oilfield, Stratum water, Chelatococcus daeguensis, HB-4, Polyphasic taxonomy

Wu Gang, Liu Yang, Li Qing, Du Huijing, You Jing, Li Hong, Ke Congyu, Zhang Xin, Yu Jiliang, Zhao Ting. Identification of Polyphasic Taxonomy for a Chelatococcus sp. HB-4[J]. Biotechnology Bulletin, 2013, 0(5): 165-170.

References

[1] Auling G, Busse HJ, Egli T, et al. Description of the Gram-negative, obligately aerobic, nitrilotriacetate (NTA) -utilizing bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov [J]. Syst Appl Microbiol, 1993, 16(1)：104-112.
[2] Yoon JH, Kang SJ, Im WT, et al. Chelatococcus daeguensis sp. nov., isolated from waste water of a textile dye works, and emended description of the genus Chelatococcus [J]. IJSEM, 2008, 58(9)：2224-2228.
[3] Panday D, Das SK. Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment [J]. IJSEM, 2010, 60(4)：861-865.
[4] 刘洋, 赵婷, 姚粟, 等.一株芝麻香型白酒高温大曲嗜热放线菌的分离与鉴定[J].生物技术通报, 2012(10)：210-216.
[5] Liu Y, Liu L, Qiu F, et al. Paenibacillus hunanensis sp. nov., isolated from rice seeds [J]. IJSEM, 2010, 60(6)：1266-1270.
[6] Chun J, Lee JH, Jung Y, et al. EzTaxon：a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences [J]. IJSEM, 2007, 57(10)：2259-2261.
[7] Thompson JD, Higgins DG, GibsonT J. CLUSTAL W：improving the sensitivity of progressive multiple sequence alignment through sequ-ence weighting, position-specific gap penalties and weight matrix choice [J]. Nucleic Acids Res, 1994, 22(22)：4673-4680.
[8] Tamura K, Dudley J, Nei M, et al. MEGA4：Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 [J]. Molecular Biology and Evolution, 2007, 24：1596-1599.
[9] 东秀珠, 蔡妙英.常见细菌系统鉴定手册[M].北京：科学出版社, 2001：399-402.
[10] 张苗, 黄少斌.高温好氧反硝化菌的分离鉴定及其反硝化性能研究[J].环境科学, 2011, 32 (1) ：259-265.
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Identification of Polyphasic Taxonomy for a Chelatococcus sp. HB-4

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