Isolation，Identification and Characteristics of Denitrifying Polyphosphate-Accumulating Organisms

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (5): 171-176.

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Isolation，Identification and Characteristics of Denitrifying Polyphosphate-Accumulating Organisms

Zhang Wugang¹, Hong Wulin², Zheng Chunli³

(1. Shanghai Tsinghua Unisplendour Environmental Engineering Co.，Ltd，Shanghai 201204；2. Shanghai Liyuan Water Treatment Technology Co.，Ltd，Shanghai 201108；3. School of Mathematics，Physics and Biological Engineering，Inner Mongolia University of Science & Technology，Baotou 014010)

Received:2012-11-10 Revised:2013-05-24 Online:2013-05-24 Published:2013-05-24

Abstract

Abstract: Being screened by a phosphate uptake experiment, where nitrates were denitrified to gas and through inspection of metachromatic and Polyphydroxybutyrate (PHB) granules, denitrifying phosphate-accumulating organisms (DNPAOs) P1-1 was isolated from a water sample in Lake Wuliangsuhai of Inner Mongolia. According to its morphological, physiological characteristics, BIOLOG identification and the analysis of its 16S rRNA sequence, it was identified as a strain of Pseudomonas fluorescens. The growth curve shows that strain P1-1 enters stationary phase in 12 hours’ training. Meanwhile, some important factors, temperature and pH value were studied to evaluate their effect on the growth and phosphorus removal ratios of strain P1-1. The results showed that the optimal conditions for the strain’s growth and phosphorus removal are 30℃ and pH8.0.

Key words: Denitrifying phosphate-accumulating organisms (DNPAOs) , Screening Identification, Temperature pH

Zhang Wugang, Hong Wulin, Zheng Chunli. Isolation，Identification and Characteristics of Denitrifying Polyphosphate-Accumulating Organisms[J]. Biotechnology Bulletin, 2013, 0(5): 171-176.

References

[1] 吕志堂, 纪翠平, 苏强, 等. 3株反硝化聚磷菌的分离与鉴定[J].环境工程学报, 2009, 3(8)：1405-1048.
[2] Lee DS, Jeon CO, Park JM. Biological nitrogen removal with enhanced phosphate uptake in a sequencing batch reactor using single sludge system [J]. Water Research, 2001, 35(16)：3968-3976.
[3] Ahn J, Daidou T, Tsuneda S, et al. Characterization of denitrifying phosphate-accumulating organisms cultivated under different electron acceptor conditions using polymerase chain reaction-denaturing gradient gel electrophoresis assay [J]. Water Research, 2002, 36(2)：403-412.
[4] Kuba T, Van Loosdrecht MCM, Heijnen JJ. Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrify dephosphatation and nitrification in a two-sludge system [J]. Water Research, 1996, 30(7)：1702-1710.
[5] Merzouki M, Bernet N, Delgenes JP, et al. Bioloical denitrifying phosphorus removal in SBR：effect of added nitrate concentration and sludge retention time [J]. Water Science and Technology, 2001, 43(3)：191-194.
[6] 马放, 王春丽, 王立立.高效反硝化聚磷菌株的筛选及其生物学特性[J].哈尔滨工程大学学报, 2007, 28(6)：631-635.
[7] 杨志愿, 邱业先, 李孝坤, 等.几株反硝化聚磷菌的筛选及其生理生化特性的鉴定[J].苏州科技学院学报, 2009, 26(3)：42-48.
[8] Merzouki M, Delgenes JP, Bernet N, et al. Polyphosphate-accumulating and denitrifying bacteria isolated from anaerobic-anoxic and anaerobic-aerobic sequencing batch reactors [J]. Current Microbiology, 1999, 3(8)：9-17.
[9] 罗宁, 罗固源, 吉方英, 等.新型双泥生物反硝化除磷脱氮系统中微生物的组成[J].给水排水, 2003, 29(8)：33-35.
[10] Buchanan RE, Gibbons NE.伯杰细菌鉴定手册(第8版)[M].北京：科学出版社, 1984：274-313.
[11] Liu JS, Xie XH, Xiao SM. Isolation of Leptospirillum ferriphilum by single-layered solid medium [J]. Journal of Central South University of Technology, 2007, 14(4)：467-473.
[12] Thompson JD, Gibson TJ, Plewniak F, et al. The CLUSTAL X windows interface：flexible strategies for multiple sequence alignment aided by quality analysis tools [J]. Nucleic Acids Research, 1997, 25(24)：4876-4882.
[13] Kumar S, Tamura K, Nei M. MEGA3：integrated software for molecular evolutionary genetics analysis and sequence alignment [J]. Briefings in Bioinformatics, 2004, 5(2)：150-163.
[14] Vlekke G, Comeau Y, Oldham WK. Biological phosphate removal from wastewater with oxygen or nitrate in sequencing batch reactors [J]. Environmental Technology Letters, 1988, 9(8)：791-796.
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Isolation，Identification and Characteristics of Denitrifying Polyphosphate-Accumulating Organisms

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