Isolation and Identification of Excellent Lactic Acid Bacteria from Silage and Its Biological Characteristics Research

Abstract

Abstract: To screen excellent microbials for silage additive usage, 12 bacteria strains were isolated from the silage. According to the results of morphological observation, physiological and biochemical tests, and sequence analysis of the 16S rRNA gene of the strains isolated, 6 strains were screened and compared through acid yield tests and bacteriostatic tests. The results showed 5 strains were Pediococcus pentosaceus, 4 strains were Lactobacillus plantarum, 1 strain was Lactobacillus fermentum, 1 strain was Leuconostoc mesenteroides subsp. mesenteroides, 1 strain was Enterococcus faecium；Strain B1-7 and B5-2 which reached a stable period after 8 h were fastest-growing, others were 14 h；The B1-7 stain no longer grow at temperature higher than 45℃, medium pH>10, salinity>0.08 g/mL. Other stains could be used as bio-straw silage additives to do further research which showed strong resistance and silage potential.

Key words: Lactic acid bacteria 16S rRNA, Screen, Biological characteristics

He Yiqun, Lei Zhaomin, Wu Run, Wan Xuerui, Diao Xiaolong, Ai Wenna. Isolation and Identification of Excellent Lactic Acid Bacteria from Silage and Its Biological Characteristics Research[J]. Biotechnology Bulletin, 2013, 0(5): 177-183.

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