Long Amplicon Real-time Quantitative PCR Technology and Its Application in DNA Damage Detection

Abstract

Abstract: DNA damage as an endpoint for evaluating genotoxicity of environmental compounds and organism levels of environmental exposure as well as the risk of tumor is always the research focus. Several methods were developed for detecting DNA damage, PCR technology, due to its little DNA needs and high accuracy is widely used in DNA damage detection recently. Here, we review the application of PCR on DNA damage detection, more over, the technical key steps of a newly developed technology called the long amplicon real-time quantitative PCR (LA-real time QPCR) also were detailed .

Key words: Long amplicon real-time quantitative, PCR DNA damage, Environmental compounds

Liang Jiao, Wang Shuhong, Zhang Ziping, Wang Yilei. Long Amplicon Real-time Quantitative PCR Technology and Its Application in DNA Damage Detection[J]. Biotechnology Bulletin, 2013, 0(5): 52-57.

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