Expression Analysis of a Banana Ubiquitin-conjugating Enzyme Gene MaUCE2 Under Abiotic Stress

Abstract

Abstract: In order to study the relationship between ubiquitin-conjugating enzyme E2 and stress resistance of plants, the banana seedings were treated with abiotic stress(drought, low temperature and salt stress). The expression levels of MaUCE2 in banana root under different stresses were analyzed by real-time qPCR. The results showed that MaUCE2 expression increased with the drought degree. In addition, the expression levels of MaUCE2 under severe drought is the highest. MaUCE2 expression is induced by low temperature stress, which increased with the decrease of the temperature. When the temperature down to 5℃, the MaUCE2 expression reached to the highest level. The expression levels of MaUCE2 under salt stress increased slightly compared with the control group. These results indicate that MaUCE2 was induced by abiotic stresses and might provide a foundation for the further research and application of biological function.

Key words: Banana, Ubiquitin conjugating enzyme, Abiotic stress Drought, Salt stress, Low temperature

Wang Anbang, Jin Zhiqiang, Liu Juhua, Jia Caihong, Zhang Jianbin, Miao Hongxia, Xu Biyu^{2, 3}. Expression Analysis of a Banana Ubiquitin-conjugating Enzyme Gene MaUCE2 Under Abiotic Stress[J]. Biotechnology Bulletin, 2013, 0(5): 77-80.

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(责任编辑李楠)