Characterization of Intestinal Microbiota in Feces from Captive Healthy Rhesus Macaques

Abstract

Abstract: With the denaturing gradient gel electrophoresis（DGGE）and real-time quantitative PCR（q-PCR）, we analyzed the predominant bacteria in fecal samples of 24 individuals divided into 6 groups. DGGE profiles showed abundant bands for the 16S rRNA gene V3 region, and the composition of the gut microbiota was apparently different in each group. Furthermore, a clustering between infant and pregnancy was shown by Principal Coordinate Analysis（PCoA）. Identified in the DGGE gels by sequencing the V3 regions were overwhelmingly affiliated with Firmicutes, Actinobacteria and Bacteroidetes. Q-PCR indicated that Clostridium and Bifidobacterium were the dominant species, and the least dominant species was Enterococcus faecali. C.clustersⅪⅤ, E.faecalis and Bacteroides-Prevotella group have the most significant diversity among individuals（P<0.01）The Bifidobacteria /Enterobacteriaceae（B/E）ratio, which indicates microbial colonization resistance（CR）in the gut appeared to be lower than the peers, subadults and adults, in pregnancy（P<0.01）. The study revealed the characterization of Rhesus Macaquesintestinal microbiota. The characteristics of infant gut microbiota are remarkably similar to those found of the pregnancy groups.

Key words: Rhesus macaques gut microbiota, DGGE Real-time quantitative, PCR

Zhao Na,Liu Shelan,Lu Jiqi,He Hongxuan,Zhao Baohua. Characterization of Intestinal Microbiota in Feces from Captive Healthy Rhesus Macaques[J]. Biotechnology Bulletin, 2013, 0(7): 153-160.

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