Cloning and Expression Analysis of Chalcone Synthase Gene from Rosa hybrida

Abstract

Abstract: It was to clone and identify chalcone synthase gene（RcCHS）from Rosa hybrida‘Fairyland’, analysis the characteristic of the sequence and expression. The total RNA was isolated from the flower petal of Rosa hybrida‘Fairyland’by improved CTAB, then reverse transcription. Using PCR degenerate primers designed based on GenBank published the conserved sequences of chalcone synthase genes from other plants to amplify cDNA fragments, a new chalcone synthase gene named RcCHS was cloned. The expression patterns of RcCHS in different developmental stages were analyzed by semi-quantitative RT-PCR. The length of RcCHS was 860 bp which encoded 287 amino acids, GenBank accession No. KC145553. Homology analysis showed that the RcCHS of Rosa hybrida‘Fairyland’had high homologies with other plants, and indicated that CHS in evolution process kept the high conservatism. RcCHS had a highest transcript level in petals at blooming stage.

Key words: Rosa hybrida RcCHS, Cloning, Expression analysis

Zhang Yunting, Yu Dingqun, Cheng Yi, Tang Haoru, Wang Qingming, Zhang Yong. Cloning and Expression Analysis of Chalcone Synthase Gene from Rosa hybrida[J]. Biotechnology Bulletin, 2013, 0(7): 66-70.

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