Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (7): 78-81.

• Study Report • Previous Articles     Next Articles

Purification and Activity Assay of Flage llin from Acidovorax avenae sub. avenae NB001

Wu Tian1，2 Liu Ying2 Yu Chao2 Bian Qiang3 Tian Fang2 Huang Qiong1 Chen Huamin2 He Chenyang2   

  1. （1. Yunnan Province Key Laboratory of Plant Pathology，Faculty of Plant Protection，Yunnan Agricultural University，Kunming 650201；2. State Key Laboratory for Biology of Plant Disease and Insect Pests，Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193；3. National Pesticide Engineering Research Center，Nankai University，Tianjin 300071）
  • Received:2013-03-22 Revised:2013-07-19 Online:2013-07-19 Published:2013-09-02

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Abstract: To elucidate the mechanism of PAMP-triggered-immunity（PTI）of rice by bacterial flagellin, a pathogen-associated molecular pattern（PAMP）, the isolation and purification of flagellin from Acidovorax avenae sub. avenae（Aaa）strain NB001 and it’s activity in elicitation of H2O2 generation of rice were performed in this study. The results showed that Aaa flagellin（about MW45 kD）was effectively purified by combined using of vortex oscillation, acetone precipitation and anion exchange chromatography. Moreover, the purified flagellin significantly induced H2O2 production in the leaf tissues of 14-day-old rice seedling. Therefore, the purified flagellin from Aaa could be further used for function analysis in PTI of rice.

Key words: Acidovorax avenae sub. avenae, Flagellin, Purification, Biological activity