Abstract:

In recent years, the researchs on assossication between the circulating microRNAs from blood and various disease or tissue damage have attracted much attention. However, the imprecision of microRNAs concentrations may be caused by cellular contamination or variance attributable to extract and process RNA. Several strategies have been proposed for normalising these causes. In this review we discuss the relative advantages and disadvantages of different normalisation strategies and instruct researchers to choose a valid normalisation strategy that will enable the measurement of biologically meaningful results.

Key words: RT-qPCR, Circulating microRNAs, Normalisation strategies, Reference genes