Analysis of Expression Pattern of LHR Gene in the Reproductive System of Yak During the Estrous Cycle

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.033

Abstract

Abstract: This experiment was conducted to study the differences in expression of luteinizing hormone receptor(LHR)gene in the reproduction of yak during the estrous cycle. Three 2-year female yaks in each phase(follicular and luteal phase)from Qinghai Province were selected for the experiment. Specific primers were designed on the basis of 5' and 3' end conservative LHR gene sequence of cattle. The comparative expression quantity of LHR gene in different phases of the reproductive system of yak was analyzed by real time quantitative PCR(RT-qPCR). The results showed that the comparative expression quantity of LHR gene in the reproductive system of yak during the estrous cycle were different, the expressions of LHR gene in ovary and oviduct were higher during the follicular phase than the luteal phase, and the expression of LHR gene in uterus was higher during the luteal phase than the follicular phase. Our findings showed that the expression of LHR gene in the reproductive system of yak during the estrous cycle was different. It implies that the LHR plays vital regulating role in the reproductive system during the reproduction of yak.

Key words: yak, LHR, reproductive system, RT-qPCR, gene expression

Hu Wei, Cui Yan, Pan Yangyang, Li Guyue, He Honghong, Xiong Li, Yu Sijiu. Analysis of Expression Pattern of LHR Gene in the Reproductive System of Yak During the Estrous Cycle[J]. Biotechnology Bulletin, 2015, 31(9): 232-237.

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