Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 58-65.

• Review • Previous Articles     Next Articles

Application of BAC-FISH in Plant Genomics Research

Gan Yimei1,Zeng Fanyun2, Zhang Shuzhen1,Yang Benpeng1,   

  1. （1. Sugarcane Research Center of Chinese Academy of Tropical Agricultural Sciences，Institute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences，Key Laboratory of Biology and Genetic Resources of Tropical Crops，Ministry of Agriculture， Haikou 571101 ；2. Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences，Haikou 571101）
  • Received:2013-04-10 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

Fluorescence in situ hybridization of bacterial artificial chromosome（BAC-FISH）is a new technology that BAC clones with different characteristics can be targeted directly to chromosomes, which plays an irreplaceable role in researches of plant genomics and molecular cytogenetics. We reviewed the application of the identification and karyotype analysis of chromosomes, the construction of genetic and physical maps, comparative mapping analysis, the origin and evolution of plants, the physical location of genes as well as the resolution and sensitivity analysis of FISH.

Key words: BAC-FISH, Plant genome, Karyotype analysis, Gene mapping Physical map