Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (2): 102-106.

• Papers • Previous Articles     Next Articles

Separation and Identification of Oviduct Glycoprotein ROGP-III from Rana chensinensis

Li Jiahui1, Liu Huimin Zhao Hongyu Liu Jingsheng   

  1. (College of Food Science and Engineering, Jilin Agricultural University,Changchun 130118)
  • Received:2013-09-09 Online:2014-02-27 Published:2014-02-27

Abstract: To study the function of the single glycoprotein in Rana chensinensis, this research determined the best coarse extraction process by orthogonal test, then purified a 66 kD single protein by salting out, dialysis, ion-exchange column chromatography and gel filtration chromatography. The purified protein identified as glycoprotein by PAS test. By HPLC identification, its purity is 97.2%, named as ROGP-III, after enzymolysis by PNGase F, it speculated that the polysaccharide content is about 17%.