Effect of Animal Endogenous Polypeptide CGA-N46 on Cell Proliferation of Candidas

Abstract

Abstract: CGA-N46 is an N terminal derived peptide of human Chromogranin A (CGA) that has anti-fungal activities. The growth inhibition effect on Candida krusei was measured with MTT assay. The cell wall integrity of Candida krusei was examined by monitoring β-1,3-glucanase activity. The effect of CGA-N46 on the permeability of Candida krusei membrane was examined using Propidium iodide detection. The effect of CGA-N46 on mitochondrial membrane potential was evaluated through Rh-123 assay. The results of showed 0.5 mg/mL CGA-N46 could significantly inhibit the proliferation of the C. krusei cells. CGA-N46 had no obvious effect on the β -1, 3-glucanase activity. There was no significant influence on the permeability of C. krusei cell membrane; CGA-N46 could significantly decrease the mitochondrial membrane potential. A conclusion can therefore be made that CGA-N46 may inhibit the growth of Candidas by affecting the mitochondrial membrane potential.

Yan Xiaohui, Li Ruifang, Zhang Huiru, Yin Yanjie, Lu Yanbo, Lu Yali. Effect of Animal Endogenous Polypeptide CGA-N46 on Cell Proliferation of Candidas[J]. Biotechnology Bulletin, 2014, 0(2): 148-152.

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