Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (2): 91-96.

• Papers • Previous Articles     Next Articles

Establishment of Agrobacterium-mediated Asiatic Lily‘Cedeazzle’Transformation System and Transfer of GsZFP1 Genes

Zhang Huan1, Zheng Jia2, Kan Dandan3, Fan Jinping1,   

  1. (1. College of Horticulture,Northeast Agricultural University,Harbin 150030;2. Personnel,Nancha area,Yichun 153000;
    3. JiJianWei,Aihui District,Heihe 164300)
  • Received:2013-08-23 Online:2014-02-27 Published:2014-02-27

Abstract: With small scales of aseptic seedling as genetic transformation, zinc finger transcription factor gene GsZFP1 was transformed into‘Cedeazzle’aseptic small scales by Agrobacterium-mediated, and the genetic transformation system of Asiatic Lily‘Cedeazzle’was established. Fifty putative transgenic plants were obtained, and 20 were identified as positive transgenic plants by PCR assay, PCR transform rate was 40%;and 12 positive plants were obtained by RT-PCR test. The results showed that GsZFP1 was expressed in Asiatic Lily‘Cedeazzle’.