The Construction of the EGFP Enterovirus 71

Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (7): 179-183.

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The Construction of the EGFP Enterovirus 71

Zhu Shouhai^{1, 2} Wang Lulu^{1, 2}

1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457；
2.Tianjin International Joint Academy of Biomedicine, Tianjin 300457

Received:2013-12-11 Online:2014-07-15 Published:2014-07-16

Abstract

Abstract: Human Enterovirus 71（EV71）is one of the major agents causing hand, foot and mouth disease（HFMD）. An infectious full-length cDNA clone of EV71 as well as the recuse virus were obtained using the reverse genetic techniques. Then, the EGFP gene was inserted into the EV71 genome to construct the reporter virus. The results show that the EGFP-EV71 virus not only are replication-competent and infectious, but the EGFP gene are stable during passaging, implicating the recombinant virus could be used as a reporter virus for high throughput EV71 antiviral drugs screening.

Key words: EV71, Infectious cDNA clone, EGFP, Reporter virus

Zhu Shouhai Wang Lulu. The Construction of the EGFP Enterovirus 71[J]. Biotechnology Bulletin, 2014, 0(7): 179-183.

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The Construction of the EGFP Enterovirus 71

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